Abstract: Objective To establish genetic monitoring method by biochemical markers for inbred gerbils and apply it in analyzing the homogenous of the ischemia-prone inbred gerbil lines CMU/1 and CMU/2. Methods Twenty-six biochemical marker loci were selected to perform cellulose acetate fiber electrophoresis for several kinds of gerbil tissues by optimum electrophoresis conditions referred to previous report and optimized the sample treatment and staining method. Then these methods were used in detecting homogeneous of 2 inbred lines CMU/1 (77 gerbils) and CMU/2 (44 gerbils) genetic quality involved generation F21-F23. Results All of 26 biochemical marker loci could be detected successfully in both inbred gerbil of 121 gerbils. Thereinto, 24 loci exhibited monomorphism within and between CMU/1 and CMU/2. However, the loci Es-3 and Es-4 showed polymorphism between two strains. Conclusion The biochemical marker method for genetic monitoring of inbred gerbil has been successfully established. The 26 biochemical marker loci for inbred gerbil strain CMU/1 and CMU/2 has been confirmed which homogeneous reached to 100%. These data indicated that two inbred strains match the standard of inbred laboratory animals (GB14923-2010).

Key words: Gerbil, Inbred strain, Ischemia model, Biochemical marker locus, Genetic monitoring