Table of Content

25 December 2017, Volume 37 Issue 6

Previous Issue    Next Issue

Therapeutic Effects of Yi Gong San Decoction on Anemia of Chronic Disease in Mouse

ZHENG Qin, JIANG Yi-ling, JI Yuting, XIA Le-ming, ZHANG Ai-ping, SHI Ling, DAI Cheng-yi, XUE Cheng, ZOU Chun-pu, LUO Mei-hong

2017, 37(6):  421-427.  DOI: 10.3969/j.issn.1674-5817.2017.06.001

Asbtract ( 269 )   PDF (13173KB) ( 116 )  

References | Related Articles | Metrics

Objective To observe the therapeutic effects of Yi Gong San decoction (YGS) on anemia of chronic disease (ACD) in mouse. Methods Totally 48 male 8-week-old male C57 BL/6 mice were randomly divided into blank control group, model group, intervention group. Each group was sacrificed 8 mouse after the continuous administration of 1 week or 2 weeks. The model group and intervention group intraperitoneally injected with zymosan A after 6 days of intraperitoneally injected with LPS. After 5 days of zymosan A injection, YGS (15.413 g per day) was administered to mice intragastrically in intervention group for successive 1 or 2 weeks while 0.2 mL of ultra-pure water was administered to mice intragastrically in blank control group and the model group. The mouse blood routine was measured by automatic cell counting. Colorimetric method was used to detect serum iron (SI) and unsaturated iron binding (UIBC). ELISA was used to detect ferritin (SF). The ratio of iron in liver and spleen were analyzed by software using Prussian blue staining. The positive area of Prussian blue iron was analyzed by Image-Pro Plus 6.0 software. The expression of hepcidin antimicrobial peptide (HAMP) mRNA in mouse liver was detected by RT-PCR. Results In the 1 or 2 weeks, the content of hemoglobin (HB), erythrocyte count (RBC), hematocrit (HCT) in mice was significantly increased (vs model group, P<0.01 or P<0.05); Hematocrit (MCV) was not changed (P>0.05). The SI and UIBC was increased (VS model group, P<0.01 or P<0.05); the SF and the ratio of iron in liver and spleen reduced significantly (VS model group, P<0.01or P<0.05). Liver HAMP mRNA was significantly decreased (P<0.01 or P<0.05). Conclusion Yi Gong San decoction can improve the degree of anemia and adjust the iron metabolism disorder in mouse of anemia of chronic disease by decreasing HAMP mRNA relative expression.



The Role of MCP-1, CD68 and ICAM-1 in the Process of Angiogenesis in Choke Area of Mice

WEN Fu-li, LIANG Cheng, ZHUANG Yue-hong, Ma Lei, ZHENG He-ping

2017, 37(6):  428-433.  DOI: 10.3969/j.issn.1674-5817.2017.06.002

Asbtract ( 259 )   PDF (15771KB) ( 128 )  

References | Related Articles | Metrics

Objective To explore the role of inflammation in angiogenesis in choke area. Methods The ear flap model of mice was established and the changes of blood vessel diameter was observed by HE staining. The expressions of intercellular cell adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1) and cluster of differentiation 68 (CD68 ) were detected by immunohistochemistry on the day after modeling(0 d), 1 d, 3 d, 5 d and 7 d, respectively. Result By observing the vascular diameter of the ear flap in mice, it was found that the vessels were visible at 3 d after operation and the vascular dilatation was obvious at 7 d. CD68-labeled macrophages had the largest accumulation in two choke area at 3 d. High expression of MCP-1 and ICAM-1 was observed in the early stage of postoperative inflammatory response. The expression of the three inflammatory factors in the tissue was increased significantly during the vascular dilation. Conclusion The three inflammatory factors (ICAM-1, CD68 and MCP-1) play an important role in vascular remodeling. Proper inflammatory response plays a positive role in flap survival.



Analysis on Genetic Diversity of Macaca mulatta littoralis by Using Microsatellite DNA Markers

ZHOU Jian-hua, LI Zhi-xiong, YANG Yan-yan, XIE Jin-dong, YU Chun-ying, WANG Xun-li

2017, 37(6):  434-441.  DOI: 10.3969/j.issn.1674-5817.2017.06.003

Asbtract ( 182 )   PDF (4232KB) ( 191 )  

References | Related Articles | Metrics

Objective To analyze the genetic diversity of Macaca mulatta littoralis and providing basic data for genetic quality monitoring methods of the population. Methods Twenty-eight Macaca mulatta littoralis were genotyped by using 20 microsatellite DNA markers, DNA was extracted from blood and amplified by PCR, and PCR products were analyzed by non-modified polyacrylamide gel electrophoresis. Every microsatellite locus was calculated by software POPGENE 1.32 and Excel. Results All 20 microsatellite loci were highly polymorphic, 200 alleles were found in the population.The number of alleles (Na), effective number of alleles (Ne), expected heterozygosity (He), observed heterozygosity (Ho), Shannon's information index (I) and polymorphism information content (PIC) were ranged from 5 to 16, 3.7333 to 11.6148, 0.7455 to 0.9305, 0.3214 to 0.7500, 1.3998 to 2.5963, and 0.6862 to 0.9074, with an average of 10, 6.8438, 0.8509, 0.4607, 2.0166 and 0.8154, respectively. All of 17 loci showed significant deviations from Hardy-Weinberg equilibrium. Conclusions There are high genetic diversity in Macaca mulatta littoralis of this colony. The experiments can provide a theoretical foundation for establishing genetic quality monitoring method with specific microsatellite loci.



Establishment and Application of Genetic Monitoring Methods for Inbred Gerbil by Biochemical Markers

LI Ying, CHEN Zhen-wen, MA Lan-zhi, SHANG Shi-chen, SHANG Yu-pu, ZHAO Quan, LI Gui-jun, CUI Xiao-xia, WANG Dong-ping, DU Xiao-yan

2017, 37(6):  442-447.  DOI: 10.3969/j.issn.1674-5817.2017.06.004

Asbtract ( 213 )   PDF (7662KB) ( 140 )  

References | Related Articles | Metrics

Objective To establish genetic monitoring method by biochemical markers for inbred gerbils and apply it in analyzing the homogenous of the ischemia-prone inbred gerbil lines CMU/1 and CMU/2. Methods Twenty-six biochemical marker loci were selected to perform cellulose acetate fiber electrophoresis for several kinds of gerbil tissues by optimum electrophoresis conditions referred to previous report and optimized the sample treatment and staining method. Then these methods were used in detecting homogeneous of 2 inbred lines CMU/1 (77 gerbils) and CMU/2 (44 gerbils) genetic quality involved generation F21-F23. Results All of 26 biochemical marker loci could be detected successfully in both inbred gerbil of 121 gerbils. Thereinto, 24 loci exhibited monomorphism within and between CMU/1 and CMU/2. However, the loci Es-3 and Es-4 showed polymorphism between two strains. Conclusion The biochemical marker method for genetic monitoring of inbred gerbil has been successfully established. The 26 biochemical marker loci for inbred gerbil strain CMU/1 and CMU/2 has been confirmed which homogeneous reached to 100%. These data indicated that two inbred strains match the standard of inbred laboratory animals (GB14923-2010).



Detection of Eimeria Stiedai by Real-Time PCR

WEN Fu-li, ZHENG He-ping, DANG Yuan, XUE Lai-en, ZHANG Shi-lan

2017, 37(6):  448-454.  DOI: 10.3969/j.issn.1674-5817.2017.06.005

Asbtract ( 245 )   PDF (9845KB) ( 139 )  

References | Related Articles | Metrics

Objective To develop the method for quantitatively detecting Eimeria stiedai (E. stiedai) by Real-Time PCR (RT-PCR). Methods Specific primers were designed and synthesized according to a part of the sequence of ITS1 region of E. stiedai. The recombinant plasmid was constructed to established the standard curve of RT-PCR. The specificity, sensitivity and reproducibility of the RT-PCR method were performed. Result The method was specific and sensitive for detection of E. stiedai. Meanwhile, the sensitivity of the present method could detect the DNA of one E. stiedai and the variation coefficients of intra-assay and inter-assay were less than 2.0% respectively. Conclusion The established RT-PCR is a specific, sensitive and reliable method for the quantitative detection of E. stiedai.



Effect of Different Treadmill Slope on Exercise Fatigue Index in SD Rats

SHI He-kun, CHEN Kai-jie, LIN Xiao-feng, YU Le, ZHU Qi-gan, FEI Yan

2017, 37(6):  455-459.  DOI: 10.3969/j.issn.1674-5817.2017.06.006

Asbtract ( 192 )   PDF (4459KB) ( 239 )  

References | Related Articles | Metrics

Objective To study the influence of exercise fatigue index on different treadmill slope in rats. Methods 32 SD rats were randomly divided into 4 groups: the control group, and 0°-, -15°- and 15°- treadmill slope exercise groups. The model group was given treadmill exercise according to the scheme. Related indexes were detected after 6 weeks training. The serum content of glucose(GLU), blood urea nitrogen(BUN), lactic acid (LA), creatine kinase (CK), hemoglobin (HGB), red blood cell (RBC). Gastrocnemius muscle index and the number of electric shock in the rats were calculated. Results Compared with the control group, there were significant changes in the blood metabolites, energy material and exercise ability during different treadmill slope exercise in the rats. The GLU, HGB and RBC in the peripheral blood samples of each exercise group were significantly lower than those in the control group (P<0.01, P<0.05), and the gastrocnemius muscle index was significantly higher (P<0.01). The serum of LA, BUN concentration and CK activity were significantly increased (P<0.01, P<0.05) in the 15°treadmill and -15°treadmill exercise groups. Compared with the 0° treadmill exercise group, the number of electric shock in the rats were significantly increased in the 15° treadmill and -15° treadmill exercise groups (P<0.05). HE staining showed that the gastrocnemius muscle cells of rats had a tendency of swelling and injury after exercise. Conclusions At 15°treadmill exercise groups, the exercise fatigue model replication of rats was better than that of 0° or -15°treadmill exercise groups.



Effects of Acrylamide on Learning and Memory Ability in Young Rats and Toxicity Alleviation by Vitamin C

LIU Xiao, XU Jing-dong, YU Fang

2017, 37(6):  460-464.  DOI: 10.3969/j.issn.1674-5817.2017.06.007

Asbtract ( 446 )   PDF (6299KB) ( 209 )  

References | Related Articles | Metrics

Objective To study the effects of acrylamide on learning and memory ability in young rats and alleviated effect of vitamin C. Methods Forty SPF grade SD rats weighing 70-90 g were randomly divided into four groups, 10 in each group and male and female in half. The control groups were given normal saline. The rats in experimental groups were given acrylamide dissolved in normal saline, at the doses of 2.5 mg/kg, 10 mg/kg and vitamin C group was given 10 mg/kg and 1 mg/kg vitamin at the same time. All animals were given test substances through oral gavage, 5 days/week, for 4 weeks. Body weight was measured per week. Special tests included the rotarod Test and the staining of rat brain tissue. Learning and memory ability was tested by Morris water maze (MWM). Results Compared with the control group, the weight of rats in high-dose group treated with acrylamide was decreased (P<0.05) and time for rats standing rotarod was decreased (P<0.05) and the latency was extended and the distance was increased by Morris water maze (MWM); The HE staining suggested that the hippocampal tissue of rats was damaged. The indexes of vitamin C group were better than the experimental groups. Conclusion Acrylamide can damage motor function and learning-memory capability in young rats and vitamin C can decrease the ACR toxicity and alleviate the hippocampal tissue damage in some degree.



Optimization on Methodology of Histopathological Examination in Adult Zebrafish Visceral Tissues

TIAN Fang, WANG Yu-zhu, XIA Min-jie, SUN Bing, DING Xun-cheng, LI Wei-hua, XU Hui-hui, HU Jing-ying

2017, 37(6):  465-469.  DOI: 10.3969/j.issn.1674-5817.2017.06.008

Asbtract ( 769 )   PDF (11345KB) ( 235 )  

References | Related Articles | Metrics

Objective To explore the optimized method of paraffin serial section and hematoxylin-eosin (HE) staining of the adult zebrafish visceral tissues. Methods After the zebrafish was fixed, the tissues were segmented, and the thoracic/abdominal segment which contains internal organs was stripped of skin and dehydrated by gradient ethanol (dehydration time was optimized to 30 min), transparented by xylene and embedded by paraffin. The tissue was sectioned serially along the body axis, rinsed with warm water after HE staining, and observed under microscope. Results The microscopic observation of heart, liver, spleen, gallbladder, kidney, pancreas, gastrointestinal tract, testis and ovary showed that effects of tissue sectioning and staining were favorable. The sections were intact without collapse or fracture, the anatomical and cytological structure of all organs were clear and complete, the nucleus were neatly blue-stained and the cytoplasm were neatly red-stained. Conclusion With the optimized histopathological method, we were able to obtain high quality histological specimens of the internal organs of adult zebrafish.



Comparison on Circle of Willis Patterns, Biochemical and Physiological Characteristics of Inbred CMU/1 and CMU/2 Gerbils

LI Ying, CUI Xiao-xia, DU Xiao-yan, MA Lan-zhi, SHANG Shi-chen, HUANG Bin, GUO Zi-xiao, ZHAO Quan, LI Gui-jun, WANG Dong-ping, CHEN Zhen-wen

2017, 37(6):  470-474.  DOI: 10.3969/j.issn.1674-5817.2017.06.009

Asbtract ( 327 )   PDF (2768KB) ( 222 )  

References | Related Articles | Metrics

Objective To compare the ratio of the incomplete circle of Willis patterns and detect the blood biochemical and physiological indexes of 2 ischemia-prone inbred gerbil lines CMU/1 and CMU/2, and to analyze the differences between 2 strains, provide reference data for gerbils experimental application. Methods Beckman automatic biochemical analyzer (CX5) and Japan photoelectric automatic blood cell analyzer (MEK-7222K) were used to determine 17 blood biochemical parameters and 22 physiological parameters. SPSS 23.0 software was used for statistical analysis by independent samples T test. Results The ratios of incomplete circle of Willis patterns in both ischemia-prone inbred gerbil lines CMU/1 and CMU/2 were more than 76%. The 12 parameters of blood physiology and biochemistry, glucose, creatinine, triglycerides, cholesterol, platelet, mean platelet volume, basophilic granulocyte, lymphocyte%, platelet distribution width, hematocrit, mean corpuscular volume, mean Corpuscular hemoglobin, were significantly different between male and female. CMU/1 and CMU/2 were significantly different in female alanine transaminase, platelet and platelet distribution width, and high-density lipoprotein cholesterol and mean corpuscular hemoglobin in males were significantly different. There were significant differences in alkaline phosphatase, alanine transaminase and aspartate transaminase between strains. Conclusion The genotypes of ischemia-prone inbred gerbil have some impact on blood biochemical and physiological parameters.

Discussing on Four diagnostic information of traditional Chinese medicine in Minipig

XIANG Bo, FAN Xiao-xue, YU Feng, LU Xue-jing

2017, 37(6):  475-477.  DOI: 10.3969/j.issn.1674-5817.2017.06.010

Asbtract ( 200 )   PDF (1133KB) ( 193 )  

References | Related Articles | Metrics

The minipig and human have many similarities.It has been widely used in animal research. In model creation of traditional Chinese medicine ,it has made a little progress, but many researchers has been plagued by the four diagnostic information collection of traditional Chinese medicine. The essay discusses collection of TCM four diagnostic information basing on looking, listening, questioning and feeling the pulse , hoping it provide useful reference for us.

Detection of Blood Physiological Parameters in Albino Mutant Golden Hamster

CUI Xiao-xia, SHANG Shi-chen, MA Lan-zhi, HUANG Bin, SHANG Yu-pu, WANG Dong-ping, CHEN Zhen-wen, WANG Quan-xin

2017, 37(6):  478-481.  DOI: 10.3969/j.issn.1674-5817.2017.06.011

Asbtract ( 229 )   PDF (1548KB) ( 194 )  

References | Related Articles | Metrics

Objective To detect the normal range of blood physiological parameters in albino mutant golden hamster and compared with wild type golden hamster. Methods Blood samples were taken from orbital venous plexus of hamsters. A number of blood physiological parameters were measured using automatic blood cell analyzer and semi-automatic coagulometer. The data was analyzed with SPSS17.0. Results The range of normal values of blood physiological data of albino mutant golden hamster and golden hamster were detected. There were significant differences between albino mutant golden hamster and golden hamster in some indexes. Conclusion The albino mutant in golden hamster may be influence the normal values of some blood physiological indexes.

P301S Mutant Tau Transgenic Mouse and Their Applications

MA Deng-lei, ZHANG Lan

2017, 37(6):  491-496.  DOI: 10.3969/j.issn.1674-5817.2017.06.015

Asbtract ( 745 )   PDF (5862KB) ( 360 )  

References | Related Articles | Metrics

Microtubule associated protein tau is the major component of the intracellular filamentous deposits of several tauopathies, including Alzheimer’s disease, frontotemporal dementia with Parkinsonism linked to tau mutations on chromosome 17(FTDP-17) and so on. Mutations in Tau as the cause of FTDP-17 have been identified in recent years. And transgenic animal models based on tau mutations have been established. Among these models, P301S transgenic mouse has been widely used in the research on tauopathies. This article reviews the research progress in pathogenic manifestations of P301S mutant tau transgenic mice and their applications.