Construction of Afp-cre-lacZ Transgenic Mouse Model

doi:10.3969/j.issn.1674-5817.2017.02.002

Abstract

Abstract: Objective To study the function of alpha-fetoprotein (Afp) positive cells during tissues growth and repair or in tumorigenesis, Afp-cre-lacZ linage tracing mouse model was established. Methods Afp-CreERT mice were established by microinjection and identified by real time PCR. Afp-CreERT mice were crossed with Rosa26-lacZ mice. Cre/lacZ both positive mice were kept as Afp-cre-lacZ mice. Results Fifty-six transgenic mice were born after microinjection. Four of them genotyped Cre positive were kept as founder. Each founder was established a strain. Cre expression of each strain was identified via real time PCR. Thus Afp-CreERT transgenic mouse was established. The Cre/lacZ both positive offspring of Afp-CreERT mice were crossed with Rosa26-lacZ mice were kept as Afp-cre-lacZ mice. Identified by real time PCR, X-gal staining and immunofluorescence, Afp-cre-lacZ mice were observed that they could be used in lineage tracing and further studies. Conclusions The Afp-cre-lacZ mice established may be used as a tool for lineage tracing studies.

Key words: Alpha-fetoprotein (Afp), Transgenic mouse model, Lineage tracing

CLC Number:

Q95-33

GU Xiao-wen, SUN Rei-lin, FEI Jian. Construction of Afp-cre-lacZ Transgenic Mouse Model[J]. Laboratory Animal and Comparative Medicine, 2017, 37(2): 89-93.

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