Improvement on Tail Tip Bleeding Model in Mice

doi:10.3969/j.issn.1674-5817.2017.01.009

Abstract

Abstract: Objective The application of tail tip bleeding model in mice was explored to evaluate the hemostatic activity of some coagulation components. Method This tail tip bleeding model improved to be used as a trauma model in the aspect of coagulant evaluation was compared with the traditional operation process. The new method was established for fast quantitative detection of the blood loss and decided the bleeding time from the starting point when the tail was cut off to the end point when the blood loss was less than 1 μL in two minutes intervals. Saculin approved as a coagulant drug on sale in domestic market was used to verify the effectiveness of this assay. The sensitivity between coagulate time and blood loss (or bleeding time) is also compared in mice. Results The advanced tail tip bleeding model in mice can quantitative analysis blood loss and bleeding time in order to reduce the personal error to a great extent. This model is easy to use and can simulate the process of trauma properly. Conclusion The tail tip bleeding model in mice can reflect the action of the coagulant drug sensitively, which is a useful tool for screening and assessment of coagulants.

Key words: Tail tip bleeding model, Mice, Simulated trauma, Blood loss, Bleeding time

CLC Number:

Q95-33

TIAN Jing, CUI Qing-hua, GUO Ping, ZHANG Zhi-xiao, FENG Zi-liang, ZHENG Ying, FAN Quan-shui. Improvement on Tail Tip Bleeding Model in Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(1): 40-45.

References

[1] Mei B, Pan C, Jiang H, et al.Rational design of a fully active, long-acting PEGylated factor VIII for hemophilia A treatment[J]. Blood, 2010, 116(2):270-279.
[2] Broze GJ Jr, Yin ZF, Lasky N.A tail vein bleeding time model and delayed bleeding in hemophiliac mice[J]. Thromb Haemost, 2001, 85(4):747-748.
[3] Maroney SA, Cooley BC, Ferrel JP, et al.Absence of hematopoietic tissue factor pathway inhibitor mitigates bleeding in mice with hemophilia[J]. Proc Natl Acad Sci U S A, 2012, 109(10):3927-3931.
[4] Tranholm M, Kristensen K, Kristensen AT, et al.Improved hemostasis with superactive analogs of factor VIIa in a mouse model of hemophilia A[J]. Blood, 2003, 102(10):3615-3620.
[5] Taylor DK.Study of two devices used to maintain normothermia in rats and mice during general anesthesia[J]. J Am Assoc Lab Anim Sci, 2007, 46(5):37-41.
[6] Flecknell P. Laboratory Animal Anaesthesia, Fourth Edition[M]. Elsevier Academic Press, 2016:50.
[7] 许火龙, 李冲, 韦晓慧, 等. 核桃叶提取液对小鼠出血时间和凝血时间的影响[J]. 中外医学研究, 2016, 14(12):2-4.
[8] 刘萍, 刘文波, 张婧, 等. 仙脉通胶囊对小鼠出血时间和凝血时间的影响[J]. 中医药信息, 2012, 29(6):40-41.
[9] 吴晓宁, 余陈欢, 徐静红. 乌蕨总黄酮及水提液的药理实验[J]. 康复学报, 2004, 14(1):13-14.
[10] Ivanciu L, Toso R, Margaritis P, et al.A zymogen-like factor Xa variant corrects the coagulation defect inhemophilia[J]. Nat Biotechnol, 2011, 29(11):1028-1033.
[11] Johansen PB, Tranholm M, Haaning J, et al.Development of a tail vein transection bleeding model in fully anaesthetized haemophilia A mice characterization of two novel FVIII molecules[J]. Haemophilia, 2016, 22(4):625-631.
[12] 祝晓雯, 宋嬿, 金若敏,等.水蛭超细粉及水煎液活血化瘀作用的比较研究[J]. 中药新药与临床药理, 2011, 22(1):33-37.
[13] 聂牧, 王云, 郭守东, 等. 板栗多糖抗动脉血栓形成的作用食品科学[J]. 2015, 36(11):187-190.
[14] Dejana E, Callioni A, Quintana A, et al.Bleeding time in laboratory animals. II - A comparison of different assay conditions in rats[J]. Thromb Res, 1979, 15(1-2):191-197.
[15] 刘彬, 张海涛, 刘慧明, 等. 鲎素对小鼠出血时间和凝血时间的影响[J]. 广东医学院学报, 2015, 33(1):49-51.
[16] 张家铨, 邹节明. 血伤宁的药理研究[J]. 中成药, 1980(2):43-46.
[17] 王继生, 李惠芝, 邱宗荫, 等. 茅莓总皂苷的主要药效学研究[J]. 中药药理与临床, 2007, 23(1):34-36.
[18] Shaw ST Jr, Aaronson DE, Moyer DL.Quantitation of menstrual blood loss-further evaluation of the alkaline hematinmethod[J]. Contraception, 1972, 5(6):497-513.
[19] Warner RL, McClintock SD, Barron AG, et al. Hemostatic properties of a venomic protein in rat organ trauma[J]. Exp Mol Pathol, 2009, 87(3):204-211.
[20] 沈稚琴, 黄稚萍. 月经血量测定的方法学和临床应用[J]. 现代检验医学杂志, 1995, 10(2):5-7.