Establishment of Preeclampsia Model in Rat and Detection on Related Indicators

doi:10.3969/j.issn.1674-5817.2015.06.004

Abstract

Abstract: Objectives To establish preeclampsia SD rat model and monitor the alterations of related indicators in conscious rats. Methods Sixteen SD rats were divided into 4 groups randomly. Telemetrical modeling group A, which was implanted radiotelemetry and built preeclampsia model. Group B was modeling control group, which only built preeclampsia model without radiotelemetry implantation. Group C was implanted radiotelemetry and operated sham-operation of preeclampsia modeling. Group D was only operated sham-operation of preeclampsia modeling. Blood pressure of rats in groups A and C were monitored continuously after radiotelemetry implantation. Urine samples were collected from each group at pregnant day 13.5 and day 20.5 for urine TP and creatinine test. Pregnant rats were euthanieed at day 21.5 of pregnancy, and then the liver, kidney and placenta tissues were collected for HE staining. Results Mean arterial pressure, systolic pressure and diastolic pressure of group A were significant higher than those of group C (P<0.01) after modeling 2 days, but at the day 21.5 of pregnancy, mean arterial pressure, systolic pressure and diastolic pressure of group A were significantly decreased compared to group C (P<0.01). In groups A and B urine TP/creatinine ratio were significantly higher than those of groups C and D(P<0.01), also their liver, kidney and placenta tissue structure get pathological changes, and 70.49% fetal growth were ceased in groups A and B. Conclusion Preeclampsia model rats have been established successfully. This rat model will contribute to research of preeclampsia.

Key words: Radiotelemetry, Reduced uterine perfusion pressure (RUPP), Preeclampsia(PE), Mean arterial pressure(MAP)

CLC Number:

Q95-33

ZHOU Jing, YAN Guo-feng, LUO Zhang-yuan, CHEN Dong-bao, CHEN Xue-jin, LI Yao. Establishment of Preeclampsia Model in Rat and Detection on Related Indicators[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 448-452.

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