Comparison on Rat Liver Tissue Slides Embedded by Paraffin among Three Different Fixatives

doi:10.3969/j.issn.1674-5817.2014.06.011

Abstract

Abstract: Objective To investigate the difference of three kinds of conventional fixatives on rat liver tissue slides embedded by paraffin and stained with HE. Methods Three kinds conventional fixatives, 10% neutral buffered formalin, Bouin's solution and modified Davidson's solution, were used to fix rat liver tissues with the animals in fasting or non-fasting conditions. The fixed tissues were embedded by paraffin, sliced and stained with HE. The qualities of the tissue slice were compared among different fixatives fixed under different conditions (fasting or non-fasting). Results Hydropic degeneration was observed in non-fasting rat liver tissue, and the liver tissue structure was fuzzy. The slices fixed by Bouin's solution and modified Davidson's solution showed clearer tissue structures, but not as brightly as the slices fixed by 10% neutral buffered formalin fixatives. Conclusion The conditions of fasting or non-fasting affect the liver tissue structure greatly. However, there is no significant difference among three kinds of fixatives fixed slices.

Key words: Rat, Liver, Tissue fixation

CLC Number:

Q95-33

ZHAO Yu-qiong, CHEN Hua. Comparison on Rat Liver Tissue Slides Embedded by Paraffin among Three Different Fixatives[J]. Laboratory Animal and Comparative Medicine, 2014, 34(6): 486-489.

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