Expression of Hypoxia Related Genes in Naked Mole Rat

doi:10.3969/j.issn.1674-5817.2014.05.012

Abstract

Abstract: Objective To detect the expression level of hypoxia related gene in lung, liver and brain of juvenile and adult naked mole rats to analyze the molecular mechanism of its resistance to hypoxia. Methods Total mRNA of the lung ,liver and brain of juvenile and adult naked mole rats were extracted respectively and then the expression of hypoxia related genes HIF-1alpha, VEGFb, FLT-1, FLT-3, Fiz1, NKRF were detected by RT-PCR. Results The expression level of HIF-1 alpha、VEGFb、FLT-1、FLT-3、Fiz1、NKRF in lung, liver and brain of adult naked mole rats was significantly higher than that of juvenile naked mole rats. Conclusion The expression level of hypoxia related genes are diferent in the naked mole rats of different ages.

Key words: Heterocephalus glaber, Tissue, Hypoxic adaptation, Related genes

CLC Number:

Q95-33

XIAO Bang, ZHAO Shan-min, LIN Li-fang, WANG Yun-hui, GONG Chen, YU Chen-lin, ZHANG Lu, TANG Qiu, SUN Wei, CUI Shu-fang. Expression of Hypoxia Related Genes in Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 400-404.

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