Effect of Bone Marrow Stem Cells on Repairment of Ileum Radiation Damage

doi:10.3969/j.issn.1674-5817.2012.03.004

Abstract

Abstract: Objective To observe the migration, colonization and repairing effects of marrow mesenchymal stem cells (MSCs) on ileum damage induced by radiation in rats. Methods The model of ileum radiation damage was made. Four rats from the ileum radiation damage model were sacrificed at 2 and 7d respectively after they were injected mesenchymal stem cells 1 ml (concentration:1 × 10⁶/ml), and ileal tissue were taken out, then the enrichment of MSCs in the ileum were observed under confocal microscope. Thirty-six rats were randomly divided into normal control group, model, and MSCs group (n=12). Animals in each group after the last injection of MSCs were sacrificed at 3 and 7 d, pathological changes of the ileum was observed. Results The occurrence of MSCs was observed in the ileum 2 d after MSC infusion, and widely distributed around the mesenteric vessels 7 d later. The intestinal structure was clear in normal control group, and epithelial cell was significantly necrosis at 3 d, involving the whole layer of cell degeneration in model group; compared with the model group, mucosal get thickness, and intestinal necrosis was relieved 7 d in treatment group. Conclusion The MSCs can be rapidly enriched in ileum radiation damage and promote regeneration and repairment.

Key words: Bone marrow mesenchymal stem cells, Ionizing radiation, Ileum, Tissue repair

CLC Number:

R329.2Q95-33

SONG Xiang-fu, SUN Bing-xue, ZHANG Jing-chun, GONG Shou-liang. Effect of Bone Marrow Stem Cells on Repairment of Ileum Radiation Damage[J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 191-194.

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