Comparative Effects of Hypoxia on Autophagy and Apoptosis of Naked Mole Rat Fibroblasts

doi:10.3969/j.issn.1674-5817.2014.05.013

Abstract

Abstract: Objective To compare the autophagy and apoptosis level of naked mole rat fibroblasts under normoxia and hypoxia. Methods The naked mole rat fibroblasts were cultured in six-well plates under hypoxic (3% O₂) or normoxia (20% O₂) conditions. The expression of Beclin 1 and LC3 were detected by western blotting and RT-PCR. Annexin V-FITC assay was used to measure the apoptotic cells by flow cytometry. Results The expression levels of LC3Ⅱ were not significantly different between hypoxic and normoxia after 24 hours. An increase was found in the LC3Ⅱ expression when comparing the two groups at 48 hour time points. As the hypoxia time prolonged, the increased expression of LC3 II protein was observed. The results of flow cytometry showed apoptosis rate of hypoxia group was significantly lower than the normal oxygen group. Conclusion Increased autophagy may protect naked mole-rat fibroblasts from hypoxia treatment, which provided insight into the research of hypoxia tolerance mechanism of naked mole rats.

Key words: Heterocephalus glaber, Skin fibroblasts, Hypoxia, Autophagy, Apoptosis

CLC Number:

Q95-33

ZHAO Shan-min, LIN Li-fang, XIAO Bang, WANG Yun-hui, GONG Chen, ZHANG Lu, YU Chen-lin, TANG Qiu, SUN Wei, CUI Shu-fang. Comparative Effects of Hypoxia on Autophagy and Apoptosis of Naked Mole Rat Fibroblasts[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 405-410.

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