Preliminary Study on Mechanism of Antiviral Effect in Naked Mole Rat

doi:10.3969/j.issn.1674-5817.2014.05.016

Abstract

Abstract: Objective To explore the pathologic changes of lung and gut of naked mole rats and the expression level of key genes of HIF-1α and NF-κB signaling pathways induced by Polyinosine-pdycytidylic acid (PolyI: C) for analyzing the possible mechanism of resistence to virus in naked mole rats. Methods The naked mole rats were divided into PolyI: C treatment group and control group (injected with normal saline). The animals were sacrificed 12 h later, and then the pathological changes of lung and gut tissue were observed. Total mRNA of lung and gut of the naked mole rats were extracted and the expression level of HIF-1α, VEGFb, VEGFc, FLT-1, Fiz1, NKRF were detected by RT-PCR. Results There is no difference in the pathological changes of lung and gut tissue between the PolyI: C treatment group and control group. The expression level of HIF-1α, VEGFb, VEGFc, FLT-1, NKRF detected were decreased significantly in treatmeat group, but the expression levels of FIZ1 in intestinal tissue increased compared to that of the control group, though the difference is not significant. Conclusion PolyI: C can not lead to obvious pathogenic changes in naked mole rats, and it can inhibit the activation of HIF-1and NF-κB signaling pathway in naked mole rats. the naked mole rats possibly inhibit the activity of signaling pathways to lead to the apoptosis of cell infected with virus to eliminate the invaded pathogens.

Key words: Heterocephalus glaber, Polyinosine-pdycytidylic acid (PolyI:C), Signaling pathway, HIF-1α, NF-κB

CLC Number:

Q95-33

XIAO Bang, ZHAO Shan-min, LIN Li-fang, WANG Yun-hui, GONG Chen, YU Chen-lin, ZHANG Lu, TANG Qiu, SUN Wei, CUI Shu-fang. Preliminary Study on Mechanism of Antiviral Effect in Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 422-427.

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