Expression of Tumor Suppressor Gene p53 in Different Tissues of Naked Mole Rat

doi:10.3969/j.issn.1674-5817.2014.05.017

Abstract

Abstract: Objective To compare the expression of tumor suppressor gene p53 in different tissues of 1-day-old naked mole rat and 1-day-old C57BL/6J mice and analyze p53 expression in different tissues of naked mole rats with different age. Methods The p53 protein expression from liver, lung, brain and kidney of newborn naked mole rats and C57BL/6J mice were determined by Western blotting. Then the p53 protein expression from liver, lung, brain and intestinal tissues from naked mole rats of different age were determined. The p53 mRNA of livers and lungs from naked mole rats were determined by RT-PCR. Exposure of cells to hypoxia increased the p53 protein expression in a time-dependent manner. Results Expression of p53 protein in the livers, lungs, brain, and intestinal tissues of the naked mole rat were lower than that in C57BL/6J mice. The expression of p53 protein in adult naked mole rat was also significantly higher than that of the young. And high expression of p53 protein was detected in the naked mole rat fibroblast cells under hypoxia. Conclusion The expression level of tumor suppressor gene p53 protein in tissues of naked mole rats was significantly lower than that of C57BL/6J mice, and significant differences of p53 expression in different tissues of naked mole rats in different tissues, which may provide autoregulation mechanism ensuring an equilibrated expression of p53 gene.

Key words: Heterocephalus glaber, Tissue, p53 gene, Hypoxia

CLC Number:

Q95-33

ZHAO Shan-min, XIAO Bang, WANG Yun-hui, LIN Li-fang, YU Chen-lin, ZHANG Lu, GONG Chen, TANG Qiu, SUN Wei, CUI Shu-fang. Expression of Tumor Suppressor Gene p53 in Different Tissues of Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 428-432.

References

[1] Buffenstein R.The naked mole-rat: a new long-living model for human aging research[J]. J Gerontol A Biol Sci Med Sci, 2005, 60(11):1369-1377.
[2] Buffenstein R.Negligible senescence in the longest living rodent, the naked mole-rat: insights from a successfully aging species[J]. J Comp Physiol B, 2008, 178(4):439-445.
[3] Seluanov A, Hine C, Azpurua J, et al.Hypersensitivity to contact inhibition provides a clue to cancer resistance of naked mole-rat[J]. Proc Natl Acad Sci U S A, 2009, 106(46):19352-19357.
[4] Tian X, Azpurua J, Hine C, et al.High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole rat[J]. Nature, 2013, 499(7458):346-349.
[5] Zheng T, Wang J, Zhao Y, et al.Spliced MDM2 isoforms promote mutant p53 accumulation and gain-of-function in tumorigenesis[J]. Nat Commun, 2013, 4:2996.
[6] 武军华, 魏文清, 贾培嫒, 等. 肿瘤抑制蛋白质P53单克隆抗体的制备及其免疫活性分析[J]. 中国药理学与毒理学杂志, 2011, 25(5):479-482.
[7] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肺脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5):378-387.
[8] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肝脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5):373-377.
[9] 赵善民, 崔淑芳. 裸鼹鼠生物学特性的研究进展[J]. 实验动物与比较医学, 2013, 33(5):400-405.
[10] 赵善民, 赵懿宁, 汤球, 等. 裸鼹鼠胸腺、脾脏及淋巴结解剖学、组织学与超微结构研究[J]. 实验动物与比较医学, 2013, 33(5):395-399.
[11] 孙伟, 汤球, 赵善民, 等. 裸鼹鼠人工饲养繁殖初步研究[J]. 实验动物与比较医学, 2013, 33(4):296-300.
[12] 邵月, 胡玉玺, 杜纪锋, 等. p53信号稳态调节线粒体能量代谢延缓衰老的运动适应[J]. 中国老年学杂志, 2009, 29(22):2966-2968.
[13] Hasty P, Vijg J.Accelerating aging by mouse reverse genetics: a rational approach to understanding longevity[J]. Aging Cell, 2004, 3(2):55-65.
[14] Rodier F, Campisi J, Bhaumik D.Two faces of p53: aging and tumor suppression[J]. Nucleic Acids Res, 2007, 35(22):7475-7484.
[15] Tyner SD, Venkatachalam S, Choi J, et al.p53 mutant mice that display early ageing-associated phenotypes[J]. Nature, 2002, 415(6867):45-53.
[16] 陈伟, 付小兵, 葛世丽, 等. 不同胎龄的胎儿和少儿皮肤中bax,bcl-2和p53基因表达的变化[J]. 中国病理生理杂志, 2005, 21(1):11-15.
[17] Matheu A, Maraver A, Klatt P, et al.Delayed ageing through damage protection by the Arf/p53 pathway[J]. Nature, 2007, 448(7151):375-379.
[18] 彭利静, 李伟, 段永宏, 等. 低氧条件培养的 PASMCs 凋亡与 p53 表达变化[J]. 第四军医大学学报, 2007, 28(16):1462-1464.