Abstract: Objective To investigate the effect of hemolysis on the value of biochemical indicators and the correlation and regression analysis between the level of hemolysis with the change of the value of biochemical indicators. Method The value of phosphorus (P), uric acid (UA), triglycerides (TG), total cholesterol (TC), total protein (TP), alkaline phosphatase (ALP), blood urea nitrogen (BUN), albumin (ALB) and glucose (GLU) in rat serum with different degrees of hemolysis wrer detected. Result Mild hemolysis has little effect on the value of biochemical indicators, while moderate and severe hemolysis affect the value measured by the biochemical analyzer of P, UA, TC, TP, ALP, BUN, ALB changes in the value of its differences between with the normal values has the statistical variation significance (P<0.05). Meanwhile, severe hemolysis impact the value of indicators of TG, and the difference was statistically significant (P<0.05). But different degrees of hemolysis has no effect of the value of GLU, the difference was not statistically significant (P>0.05). Conclusion Different levels of hemolysis have different effects on conventional values of biochemical indicators, and different regression equations can be used to correct the value of different biochemical markers.

Key words: Hemolysis, Rat, Biochemical indicators, Correlation analysis