Effects of Sleep Deprivation on Rat Liver Iron Metabolism

doi:10.3969/j.issn.1674-5817.2014.03.007

Abstract

Abstract: Objective To explore the influence of sleep deprivation on rat liver iron metabolism and related molecular mechanism. Methods Twenty Sprague-Dawley male rats (weight about 180 g) were randomly divided into normal control group (CG) and sleep deprivation group (SDG). The SD model was induced in rats by improved multi-platform sleep deprivation method. After 5 days’ sleep deprivation, the colorimetry was used to detect the iron ion concentration in rat’s serum and liver tissue samples; the expression of HAMP, IL-6 and IL-1 mRNA in rat’s liver was observed by real-time fluorescent quantitative PCR. The expression of hepatic ferritin and STAT3 protein was detected by Western Blot. Results Compared with CG group, serum iron level in SDG rats reduced (P<0.05), while liver iron concentration was higher (P<0.01); the expression of HAMP, IL - 6 and IL - 1 mRNA in SDG liver tissue increased by 1.1, 3.2 and 1.7 times respectively (P<0.01); the expression of ferritin and phosphorylated STAT3 protein in SDG liver tissue significantly higher than control group (P<0.01). Conclusion Sleep deprivation disturb iron metabolism in rats through IL - 6 - hepcidin axis.

Key words: Sleep deprivation, Liver, Iron metabolism, Hepcidin, Interleukin-6

CLC Number:

Q95-33

GUAN Yu, YAN Xiao-li, WANG Guo-hua, ZHANG Xiao-feng. Effects of Sleep Deprivation on Rat Liver Iron Metabolism[J]. Laboratory Animal and Comparative Medicine, 2014, 34(3): 205-209.

References

[1] 张亚晶, 卢才义. 睡眠剥夺对心血管系统的影响及其研究进展[J]. 中华老年多器官疾病杂志, 2008, 7(6):529-532.
[2] Goel N, Basner M, Rao H, et al.Circadian Rhythms, Sleep Deprivation, and Human Performance[J]. Prog Mol Biol Transl Sci, 2013, 119: 155-190.
[3] O’Keeffe ST, Gavin K, Lavan JN. Iron status and restless legs syndrome in the elderly[J]. Age Ageing, 1994, 23(3):200-203.
[4] Suehecki D, Tufik S.Social stability attenuates the stress in the modified multi Platform method for Paradoxical sleep deprivation in the rat[J]. Physiol Behav, 2000, 68(3):309-316.
[5] Zhang Z, Zhang F, An P, et al.Ferroportin1 deficiency in mouse
macrophages impairs iron homeostasis and inflammatory responses[J]. Blood, 2011, 118(7):1912-1922.
[6] Trenkwalder C, Paulus W.Restless legs syndrome: pathophysiology, clinical presentation and management[J]. Nat Rev Neurol, 2010, 6(6):337-346.
[7] Allen R.Dopamine and iron in the pathophysiology of restless legs syndrome (RLS)[J]. Sleep Med, 2004, 5(4): 385-391.
[8] Meynard D, Babitt JL, Lin HY.The liver: conductor of systemic iron balance[J]. Blood, 2014, 123(2):168-176.
[9] Carbia-Nagashima A, Arzt E.Intracellular proteins and mechanisms involved in the control of gp130/JAK/STAT cytokine signaling[J]. IUBMB Life, 2004, 56(2):83-88.
[10] Chuang PY, He JC.JAK/STAT signaling in renal diseases[J]. Kidney Int, 2010, 78(3):231-234.
[11] Goebel MU, Mills PJ, Irwin MR,et al.Interleukin-6 and tumor necrosis factor-alpha production after acute psychological stress, exercise, and infused isoproterenol: differential effects and pathways[J]. Psychosom Med, 2000, 62(4):591-598.
[12] Ali T, Choe J, Awab A, et al.Sleep, immunity and inflammation in gastrointestinal disorders[J]. World J Gastroenterol,2013, 19(48):9231-9239.
[13] Vgontzas AN, Papanicolaou DA, Bixler EO, et al.Circadian interleukin-6 secretion and quantity and depth of sleep[J]. J Clin Endocrinol Metab, 1999, 84(8): 2603-2607.
[14] Nemeth E, Valore EV, Territo M, et al.Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein[J]. Blood, 2003, 101(7):2461-2463.
[15] Zhao M, Chen J, Wang W, et al. Psychological stress induces hypoferremia through the IL-6-hepcidin axis in rats[J]. Biochem Biophys Res Commun, 2008, 373(1):90-93.