Establishment of Nonalcoholic Fatty Liver Fibrosis Model and Expression of Inflammatory Factors in Mice

doi:10.3969/j.issn.1674-5817.2017.01.005

Abstract

Abstract: Objective To establish the non-alcoholic fatty liver fibrosis model in mice and observe the pathological changes and the expression of inflammatory factors. Methods Twenty male C57BL/6J mice were randomly divided into control group and model group. The mice in control group were fed with methionine and choline-supplement(MCS) diet, and the model group were fed with methionine choline deficiency (MCD) diet. The mice were weighed and sacrificed at after 8 weeks feeding. The histopathological changes in liver were observed. The level of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol (TC), triglyceride (TG), tumor necrosis factor -〈 (TNF-〈) and interleukin (IL-6 ) were detected. Results The body weight of mice in the model group decreased significantly at the end of the study. Severe hepatic steatosis, liver cell edema, visible focal necrosis and focal lymphocytic infiltration, interface hepatitis, periportal enlargement, hyperplasia of fibrous tissue were observed.The scores of liver inflammation activity and fibrosis score in model group were significantly higher than those in control group (P<0.01).The level of serum ALT、AST、TNF-〈、IL-6 were significantly higher in the model group than that of control group (P<0.01). Conclusion After feeding mice with MCD diet feeding for 8 weeks, non-alcoholic fatty liver fibrosis model of mice was successfully induced. The modeling method was simple, rapid, and with has high survival rate. It is suitable for the pathogenesis and drug intervention study of nonalcoholic fatty liver fibrosis.

Key words: Non-alcoholic fatty liver Fibrosis, Methionine choline deficiency(MCD), C57BL/6J mice, Inflammatory factors

CLC Number:

Q95-33

YANG Hua, ZHAO Ya-Juan, OU Qiang. Establishment of Nonalcoholic Fatty Liver Fibrosis Model and Expression of Inflammatory Factors in Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(1): 20-24.

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