Abstract: Objective To observe the therapeutic effects of Yi Gong San decoction (YGS) on anemia of chronic disease (ACD) in mouse. Methods Totally 48 male 8-week-old male C57 BL/6 mice were randomly divided into blank control group, model group, intervention group. Each group was sacrificed 8 mouse after the continuous administration of 1 week or 2 weeks. The model group and intervention group intraperitoneally injected with zymosan A after 6 days of intraperitoneally injected with LPS. After 5 days of zymosan A injection, YGS (15.413 g per day) was administered to mice intragastrically in intervention group for successive 1 or 2 weeks while 0.2 mL of ultra-pure water was administered to mice intragastrically in blank control group and the model group. The mouse blood routine was measured by automatic cell counting. Colorimetric method was used to detect serum iron (SI) and unsaturated iron binding (UIBC). ELISA was used to detect ferritin (SF). The ratio of iron in liver and spleen were analyzed by software using Prussian blue staining. The positive area of Prussian blue iron was analyzed by Image-Pro Plus 6.0 software. The expression of hepcidin antimicrobial peptide (HAMP) mRNA in mouse liver was detected by RT-PCR. Results In the 1 or 2 weeks, the content of hemoglobin (HB), erythrocyte count (RBC), hematocrit (HCT) in mice was significantly increased (vs model group, P<0.01 or P<0.05); Hematocrit (MCV) was not changed (P>0.05). The SI and UIBC was increased (VS model group, P<0.01 or P<0.05); the SF and the ratio of iron in liver and spleen reduced significantly (VS model group, P<0.01or P<0.05). Liver HAMP mRNA was significantly decreased (P<0.01 or P<0.05). Conclusion Yi Gong San decoction can improve the degree of anemia and adjust the iron metabolism disorder in mouse of anemia of chronic disease by decreasing HAMP mRNA relative expression.

Key words: Anemia of chronic disease (ACD), Yi Gong San decoction (YGS), Treatment, Hepcidin