Expression of nm23-M1 Gene in Mouse Myelomonocytic Leukemia

doi:10.3969/j.issn.1674-5817.2014.01.008

Abstract

Abstract: Objective To investigate the expression level of nm23-M1 gene in mouse myelomonocytic leukemia. Methods The mice were inoculated intravenously with myelomonocytic leukemia cells of WEHI-3 cells, and selected a control group of normal mice. Bone marrow samples were collected in two groups. The nm23-M1 mRNA expression were detected by TaqMan quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR) and analysis of its relationship with the occurrence and development of leukemia. Results The expression of nm23-M1 gene in bone marrow cells of mice with leukemia is 24 times of the normal control group. There was a significant difference between the two groups (P<0.05). Conclusion The nm23-M1 gene overexpressed .in mouse myelomonocytic leukemia, and had close relationship between and peripheral white blood cell count, the number of original cells in bone marrow and organ against the leukemia cases, regardless of the gender, age and leukemia in mice.

Key words: nm23-M1 gene, Myelomonocytic leukemia

CLC Number:

Q95-33

DONG Zheng, SUN Xue-dong, MAN Qiu-hong, YAO Bo, LI Yu-fang, LIU Tie-qiang, HUANG Shan, LIU Gguang-xian, Ai Hui-sheng, GUO Mei. Expression of nm23-M1 Gene in Mouse Myelomonocytic Leukemia[J]. Laboratory Animal and Comparative Medicine, 2014, 34(1): 42-45.

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