Comparative Study on Autophagy Regulation between Naked Mole Rat and C57BL/6 Mouse

doi:10.3969/j.issn.1674-5817.2013.04.011

Abstract

Abstract: Objective A comparative study between naked mole rat and C57BL/6 mouse on autophagy regulation is conducted, providing a reference for the research of mechanism in high autophagy, anti-cancer, slow aging of NMR. Methods Electron microscopy and western blotting are use to detect the activtity of autophagy in the liver and lung tissue of newborn naked mole rat and C57BL/6. Reagents interfere the autophagy level of fibroblasts derived from naked mole rat and mouse. The cell number is tested after 24, 48, 72 hours by CCK-8 kit. Meanwhile, expression of Beclin 1 at each time point is detected by western blotting. Results Compared with mice, the naked mole rat tissue have significantly higher LC3B expression. The NMR cells have a certain amount of resistance from 3-MA. Rapamycin’s proliferation inhibition on NMR-S(fibroblasts derived from skin of naked mole rats)was significantly greater than that of mouse cells. Conclution NMR tissue possess a high autophagy, Cells from NMR have greater ability of self-regulation under extraneous autophagy inhibition, compared to C57BL/6.

Key words: Naked mole rat, C57BL/6 mouse, Autophagy regulation, Comparative study

CLC Number:

Q95-33

LIN Li-fang, ZHAO Yi-ning, ZHAO Shan-min, XU Chen, CUI Shu-fang. Comparative Study on Autophagy Regulation between Naked Mole Rat and C57BL/6 Mouse[J]. Laboratory Animal and Comparative Medicine, 2013, 33(4): 301-305.

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