A Preliminary Study on Effects of Lipopolysaccharide on Lung Tissues of Naked Mole Rats

doi:10.3969/j.issn.1674-5817.2020.06.010

Abstract

Abstract: Objective To detect the effects of lipopolysaccharide (LPS) on lung morphology and pathological parameters, and explore the LPS tolerance in naked mole rats. Methods Ten male naked mole rats were randomly divided into an experimental group and a control group. The naked mole rats of the experimental group were injected with LPS at 10 mg/kg according the body weight, while the naked mole rats of the control group were injected with the same volume of normal saline. Six hours after the administration, lung tissues were collected to detect the ratio of wet to dry weight; paraffin sections were prepared and stained with HE, and the lung microstructure was observed under optical microscope; the level of reactive oxygen species (ROS) and the apoptosis ratio of lung tissue cells were detected by flow cytometry. Results Microscopic examination showed that the lung tissue structure of naked mole rats in the experimental group was intact, and there were no obvious exudate and inflammatory cell infiltration in the alveolar foramen. Compared with the control group, the ratio of wet to dry weight of lung tissues in the experimental group and the proportion of early apoptosis had no significant differences, but the proportion of late apoptosis and the content of ROS increased significantly (P < 0.01, and P < 0.05, respectively). Conclusion Naked mole rats can tolerate the lung injury induced by LPS to some degree, and the specific mechanism needs to be further studied.

Key words: Naked mole rats, Lung, Lipopolysaccharide

CLC Number:

Q95-33

ZHANG Chengcai, LIU Yinhang, CHEN Chao, JIANG Wenzheng. A Preliminary Study on Effects of Lipopolysaccharide on Lung Tissues of Naked Mole Rats[J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 523-527.

Figures/Tables 2

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