Laboratory Animal and Comparative Medicine ›› 2013, Vol. 33 ›› Issue (4): 306-309.DOI: 10.3969/j.issn.1674-5817.2013.04.012

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A Report on Breeding of GFP Nude Mouse

ZHANG Li-bo, Liu Biao, TIAN Xiao-yun, WU Zheng-lin, ZHOU Sen-mei, YOU Jin-wei, FANG Tian, YUN Shi-feng   

  1. Nanjing General Hospital of Nanjing Military Command Comparative Medicine Department Nanjing 210002 China
  • Received:2012-10-29 Online:2013-08-25 Published:2013-08-25

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Abstract: Objective To breed a strain of BALB/c nude mice with green fluorescence and to observe the expression of green fluorescent protein in various organs. Method The nude mice expressing stable green fluorescence were developed by hybridizing, self-breeding and backcrossing BALB/c-nu/nu male mice with C57BL/6-TgN (GFP) female mice according to the principles of genetics. The genetic quality, thymus growth and green fluorescent protein expression in multiple organs were observed. Result The GFP nude mouse in line with BALB/c mouse was successfully bred. The mouse were presented a yellow-green appearance and characterized by athymism. The green fluorescence can be seen clearly in major organs such as liver, kidneys and pancreas under a fluorescent microscope. Conclusion A BALB/c GFP nude mouse model is developed successfully, its appearance is yellow-green and its whole-body expresses the green fluorescent protein stably.

Key words: Green fluorescent, Nude mouse, Breeding, Expression

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