Influence of Cobalt Chloride Induced Hypoxia on Proliferation and Apoptosis of Hepatic Stellate Cells in Naked Mole Rat

doi:10.3969/j.issn.1674-5817.2016.01.014

Abstract

Abstract: Objective To compare and study the influence of cobalt chloride (CoCl₂) on proliferation and apoptosis of hepatic stellate cells in the naked mole rat and C57BL/6J mouse, and preliminarily investigate the possible mechanism of hypoxic tolerance in naked mole rat. Methods Evaluating the effects of different concentrations of CoCl₂ on proliferation activity and apoptosis level of hepatic stellate cells in the naked mole rat and mouse by Cell Counting Kit-8 assay (CCK8) and flow cytometry. Detect the expression of hypoxia inducible factor 1〈 (HIF-1〈) and apoptosis-related proteins (BCL₂, BAX)of hepatic stellate cells in the naked mole rat after CoCl₂ treatment by Western blot assay. Results CoCl₂significantly inhibited the proliferation and increased the apoptotic rate of hepatic stellate cells in C57BL/6j mice at a low concentration of 50 μmol/L. However, a same dose of CoCl₂ can significantly improve the proliferation of hepatic stellate cells in the naked mole rat but had no significant effect on its apoptotic rate. While high concentrations of CoCl₂ (> 200 μmol/L) showed a inhibition of proliferation and a rise of apoptotic rate of hepatic stellate cells in naked mole rat and mice, but the CoCl₂-induced changes in hepatic stellate cells of the naked mole rat were much smaller than those of mice. Simultaneously, the expression of HIF-1〈 and the ratio of apoptosis-related proteins (BCL₂/BAX) in hepatic stellate cells of the naked mole rat upregulated or accumulated significantly after treating with CoCl₂ (P<0.05). Conclusion Compared with C57BL/6J mice, the naked mole rat hepatic stellate cells had a greater ability of resistance to hypoxic damage caused by CoCl₂. It was speculated that HIF-1〈 may be involved in the regulation of resistance to damage caused by hypoxic environment in naked mole rat.

Key words: Cobalt chloride(CoCl₂), Naked mole rat, Hepatic stellate cells, Apoptosis

CLC Number:

Q95-33

XIAO Bang, LI Li, YU Chen-lin, ZHAO Shan-min, LIN Li-fang, YANG Wen-jing, CONG Wei, CUI Shu-fang. Influence of Cobalt Chloride Induced Hypoxia on Proliferation and Apoptosis of Hepatic Stellate Cells in Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2016, 36(1): 66-71.

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