The Effect of Poly I:C on Autophagy of Naked Mole Rat

doi:10.3969/j.issn.1674-5817.2014.05.014

Abstract

Abstract: Objective To study the effect of polyinosinic-polycytidylic acid(Poly I: C) on the autophagy in naked mole rat. Methods The adult naked mole rats and C57BL/6 mice were randomly divided into Poly I: C treatment group (ip Poly I: C) and control group (ip saline). Twelve hours after Poly I: C administered, the differences in the intestinal pathological changes and subcellular organelles between naked mole rats and C57BL / 6 mice were observed by light and electron microscopy respectively. The mRNA expression of inflammation-related factor IL-1 and autophagy-related gene ATG5 in intestinal tissue were detected by quantitative real-time PCR. Detection of autophagy-related proteins LC3B and Beclin 1 was conducted by Western blot. Results Naked mole rats did not show significant pathological changes after Poly I: C treatment, but intestinal tissue glands of C57BL/6 mouse show serious hemorrhage. Further observation by electron microscope found that the mitochondria’s size was elongagted, number increased and more autophagy in naked mole rats’ intestinal tissue cells. Meanwhile, a lot of vacuolatation of mitochondria, dilation of smooth endoplasmic reticulum was observed in mouse intestinal tissues. Significant difference was not found in mRNA expression of IL-1, ATG5 by real-time quantitative PCR. In Western blot detection, LC3B protein expression was significantly increased (P<0.05) in naked mole rats, after administration, but that of C57BL/6 mice were reduced (P<0.05). Conclusion Poly I: C administration induced upregulation of autophagy in naked mole rat. While, autophagy may regulate the inflammatory response in turn, which played an important role in naked mole rats’ anti-Poly I:C injury.

Key words: Naked mole rat, polyinosinic-polycytidylic acid(Poly I: C), Inflammation, Autophagy

CLC Number:

Q95-33

LIN Li-fang, XIAO Bang, ZHAO Shan-min, TANG Qiu, YU Chen-lin, SUN Wei, ZHANG Lu, CAI Li-ping, XU Chen, CHENG Ji-shuai, CUI Shu-fang. The Effect of Poly I:C on Autophagy of Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 411-416.

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