Establishment of Method for Separation, Cultivation of Bone Marrow Macrophage in Naked Mole Rats and Preliminary Research on Function

doi:10.3969/j.issn.1674-5817.2016.01.015

Abstract

Abstract: Objective To establish the method for the primary culture of bone marrow macrophage in naked mole rats and study the phagocytosis compared with mice. Method The bone marrow cells from the hing leg of naked mole rats were detached. The cells were induced by 60 ng/mL Macrophage colony-stimulating factor (M-CSF) and cultured for 6~7 days in vitro. During the culture, the cell was observed in microscope. After induced, the flow cytometry was conducted to detect the purity of macrophage and the phagocytosis of macrophage with mouse as control. Result Through this method, the adherent cells with typical morphological characteristics were obtained and the positive rate of the cell surface antigen F4/80 and CD11b were higher than 80%. The phagocytosis rate of naked mole rats bone marrow macrophage was 99.87%±0.13%, but the ICR mice was 88.79%±0.90%. Conclusion This method is simple and practical for separation and culture of highly purified and functional naked mole rats bone marrow macrophage in vitro. It indicates that phagocytosis of naked mole rats bone marrow macrophage is higher than that of mice.

Key words: Naked mole rat, Bone marrow macrophage, Separation and cultivation, Function

CLC Number:

Q95-33

CHENG Ji-shuai, LI Li, XIAO Bang, ZHAO Shan-min, LIN Li-fang, CONG Wei, TANG Qiu, SUN Wei, YU Chen-lin, YANG Wen-jing, XU Chen, CUI Shu-fang. Establishment of Method for Separation, Cultivation of Bone Marrow Macrophage in Naked Mole Rats and Preliminary Research on Function[J]. Laboratory Animal and Comparative Medicine, 2016, 36(1): 72-75.

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