Detection on Level of Immune-related Factors mRNA in Peripheral Blood Leucocyte of Naked Mole Rats’

doi:10.3969/j.issn.1674-5817.2014.05.015

Abstract

Abstract: Objective To study the normal immunity function level of naked mole rats in growth period and adulthood, detection on mRNA expression levels of immune-related factor was conducted in 6-month and 12-month-old naked mole rats’ peripheral leukocytes. Methods The peripheral blood was collected from orbital venous plexus in 6-months and 12-months-old naked mole rat. Total mRNA was extracted from peripheral blood leukocytes, and reversed into cDNA. Then, mRNA expression levels of IL-1, IL-2, IL-4, IL-6, IL-8, IL-12, TNF-α, IFN-γ was tested by real-time quantitative PCR. Results Apart from IFN-γ, IL-4, which is no significant difference between the groups, the mRNA expression of IL-2, TNF-α of 6-month-old naked mole rats were significantly higher than those of 12-month-old, especially the 6-month-old male group. The expression of IL-1, IL-6 in 6-month-old male group was also significantly higher than those of the 12-month-old groups. In addition, 12-month-old female group had the lowest IL-8 level, and 12-month-old male naked mole rats had the lowest IL-12 level. Conclusion The male naked mole rats in growing period had the higher immune level than their even-aged female and adults, whether in humoral or cellular immunity.

Key words: Naked mole rat, Immune-related factors, Normal immune level, Interleukin

CLC Number:

Q95-33

LIN Li-fang, XIAO Bang, ZHAO Shan-min, TANG Qiu, YU Chen-lin, SUN Wei, ZHANG Lu, CAI Li-ping, XU Chen, CHENG Ji-shuai, CUI Shu-fang. Detection on Level of Immune-related Factors mRNA in Peripheral Blood Leucocyte of Naked Mole Rats’[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 417-421.

References

[1] Buffenstein R.Negligible senescence in the longest living rodent, the naked mole-rat: insights from a successfully aging species[J]. J Comp Physiol B,2008,178(4):439-445.
[2] Liang ST, Mele J, Wu Y, et al.Resistance to experimental tumorigenesis in cells of a long-lived mammal, the naked mole-rat (Heterocephalus glaber)[J]. Aging Cell, 2010, 9(4):626-635.
[3] Buffenstein R.The naked mole-rat: a new long-living model for human aging research[J]. J Gerontol A Biol Sci Med Sci,2005, 60(11):1369-1377.
[4] Yamamura M, Modlin RL, Ohmen JD, et al.Local expression of antiinflammatory cytokines in cancer[J]. J Clin Invest,1993, 91(3):1005-1010.
[5] 张璐, 赵懿宁, 袁子彦, 等. 裸鼹鼠与C57BL/6J小鼠肾脏组织结构及超微结构比较[J]. 实验动物与比较医学, 2013, 33(5):388-394.
[6] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肺脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5):378-382.
[7] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肝脏显微结构与超微结构观察[J].实验动物与比较医学, 2013, 33(5):373-377.
[8] 赵善民, 孙伟, 汤球, 等. 裸鼹鼠血液生理生化及尿常规值的测定[J]. 实验动物与比较医学, 2013, 33(6):469-472.
[9] 林丽芳, 王洪, 赵善民, 等. 裸鼹鼠生化位点的初步研究[J].实验动物与比较医学, 2013, 33(6):477-480.
[10] 林丽芳, 赵懿宁, 赵善民, 等. 裸鼹鼠与C57BL/6小鼠自噬调节的比较研究[J].实验动物与比较医学, 2013, 33(4):301-305.
[11] Zhao SM, Lin LL, Kan GH, et al.High autophagy in the naked mole rat may play a significant role in maintaining good health[J].Cell Physiol Biochem, 2014, 33(2):321-332.
[12] Bubenik J.MHC class I down-regulation: tumour escape from immune surveillance? (review)[J].Int J Oncol,2004,25(2):487-491.
[13] Salgaller ML, Lodge PA.Use of cellular and cytokine adjuvants in the immunotherapy of cancer[J]. J Surg Oncol, 1998,68(2):122-138.
[14] Wong RJ, Patel SG, Kim S, et al.Cytokine gene transfer enhances herpes oncolytic therapy in murine squamous cell carcinoma[J].Hum Gene Ther, 2001, 12(3):253-265.
[15] Younes A, Kadin ME.Emerging applications of the tumor necrosis factor family of ligands and receptors in cancer therapy[J].J Clin Oncol,2003,21(18):3526-3534.
[16] Bickel M.The role of interleukin-8 in inflammation and mechanisms of regulation[J]. J Periodontol, 1993, 64(Suppl 5):456-460.
[17] 卢守明, 卢守亮, 孙天威, 等. 雌激素干预去势骨质疏松症大鼠血清白细胞介素8、10的表达[J]. 中国组织工程研究, 2013, 17( 24): 4394-4400.
[18] 臧鲁心, 马保臣, 董玉兰, 等. 妊娠期激素对 Th1和 Th2细胞因子的调控[J].动物医学进展, 2006, 27(1): 24-27.
[19] 郑国强, 刘安军, 滕安国, 等. 雌雄小鼠胸腺和脾脏的免疫比较[J]. 安徽农业科学, 2011, 39(16):1-6.
[20] 崔淑芳, 陈学进, 汤球, 等. 实验动物学[M]. 3版. 上海:第二军医大学出版社, 2013:146-147.