Abstract: Objective A micro-surgery model through femoral arterial and gastric cannula in mice is introduced. Methods After mice were anesthetized by inhalation of isoflurane, catheters were placed into the femoral artery. Thereafter, gastric cannula was tunneled subcutaneously through two incisions between the back of the neck and the left upper quadrant. After cutting through the abdominal muscle, the stomach was exposed. Gastric cannula was then implanted into the lower end of the stomach through an incision on the gastric wall. Heparin (20 u,12~15 µl/h) were continuously infused in both catheters through a double-channel swivel connected with a pump. Result All mice fully recovered after surgeryŁ¨1ˇ«2 daysŁ©. The artery catheters and gastric cannula work well until 7 day after micro-surgery operation. Wound infection, gastric fistula and peritoneal abscess were not found. Conclusion This is a dynamic, safe, as well as efficient micro-surgery model, with less injury to the mice and smaller foreign matter in the mouse stomach. It will be a useful model for pharmacological, toxicological, and clinical experimental studies.

Key words: Mice, Femoral artery catheter, Gastric cannula, Micro-surgery model