Abstract: Objective To establish the animal model of diabetic rats with fluctuant high blood glucose and observe the effects of fluctuant high blood glucose on pancreas in diabetic rats. Methods The diabetic rats model was developed by injection with streptozotocin (STZ) 30 mg/kg, regularly staggered insulin 1U and glucose 0.375 mg/kg administration were used to set up blood glucose fluctuation model. General symptoms were observed and the data of FFA、INS、HbAlc in blood as well as the Bax and Bcl-2 in pancreas were measured at the thirteen week. Results (1) The typical diabetic symptoms in blood fluctuation group and diabetic group were more obvious than those in normal group. (2) The blood glucose levels in diabetic group and blood glucose fluctuation were significant higher than that in normal group. (3) The FFA、HbAlc、INS in diabetic group and blood glucose fluctuation group were higher than those in normal group (P<0.01); The FFA、HbAlc、INS in blood glucose fluctuation group was higher than those in diabetic group (P<0.01). (4) Compared with normal group, the Bax in pancreas was obviously increased (P<0.01) and the Bcl-2 was dramatically reduced in blood fluctuation group and diabetic group (P<0.01); The Bax was higher (P<0.01) and the Bcl-2 was lower (P<0.01) in blood fluctuation group than those in diabetic group. Conclusion The blood fluctuation diabetic rats model was successfully established by the method of subcutaneous insulin and intraperitoneal glucose injection in diabetic rats twice daily, and fluctuant high blood glucose aggravates the injury of the pancreas in diabetes rats.

Key words: Diabetes mellitus, Blood glucose fluctuation, Model, Pancreas