Abstract: Objective To explore methods to establish different models infected with methicillin resistant Staphylococcus aureus (MRSA). Methods Forty-eight specific pathogen free SKH-1 hairless mice were randomly divided into 4 groups. Mice from the PBS control groups were treated with sterilized PBS, by subcutaneous injection and direct daub on the puncture of skin injury by needle respectively, mice from model groups were treated with MRSA (ST-239) by subcutaneous injection or direct daub on the puncture of skin injury by needl. Models were evaluated by appearance and volume of abscesses or ulcers, CFU extracted from skin and morphology. Results There were significant differences in appearance, volume and CFU extracted from skin between the two models. Evident pathological alterations were observed. Skin of mice from subcutaneous injection group developed abscesses with limited inflammation, with no abscesses and diffuse inflammation induced in needle injury group. Conclusions Mice treated with MRSA by subcutaneous injection or diret daub on the puncture of skin injury by needl can develop abscesses model and wound-infected model respectively.

Key words: Methicillin resistant Staphylococcus aureus(MRSA), Skin infection, Model, SKH-1 hairless mice