Study on BMP-2 on Inducing OPG-/-BMSCs Differentiate into Osteogenesis in vitro

doi:10.3969/j.issn.1674-5817.2012.03.003

Abstract

Abstract: Objective To investigate the effect of bone morphogenetic protein-2(BMP-2) on inducing OPG^-/- BMSCs differentiate into the osteogenesis in vitro, and to evaluate the effect of BMP-2 Bioactive Peptides inducing to osteoplast and dose dependent. Methods Got the femur of mice, separated the BMSCs and cultured in vitro. Followed by passaged the BMSCs to P1 using the conditioned medium, and then the BMSCs were added in the BMP-2 consistency of 0.3 μg、0.2 μg、0.1 μg、0.05 μg、0 μg separately cultured with 15% FBS culture medium, marked A, B, C, D, and E group in order. The 3^rd, 5^th, 7^th day after cell culture, the expression of alkaline phosphatase was detected by PNPP method, and calcium content was detected by Calcium kit. The expression of tatrate-resistant acid phosphatase form 5 b in the cell supernatant detected by ELISA, To investigate the effect of different concentration bone morphogenetic protein-2 (BMP-2) on inducing OPG^-/- BMSCs differentiate into the osteogenesis in vitro. Results The activity of alkaline phosphatase and calcium content showed that group A and group B both increase higher than the other group (P<0.05). However no significant differences were detected between the group A and B (P>0.05). TRACP5b displayed, TRACP5b expression in group A, B and C is lower than the other groups in the 3^rd, 5^th, 7^th day (P<0.05), no significant differences were detected between the group A and B (P>0.05). Conclusions The BMP-2 had significant effect on inducing OPG^-/- BMSCs differentiate into the osteogenesis in vitro. The effect dependent on the different concentration of bone morphogenetic protein-2(BMP-2), the optimal concentration is 0.2 μg/ml.

Key words: Bone morphogenetic protein-2(BMP-2), OPG^-/- mouse, Bone marrow derived stroma cell

CLC Number:

Q95-33

ZHANG Chun, WEI Qin, WANG Xue-mei, KUANG Ying, DUAN Ming-jun, ZUOFEIYA·Keyimu, JIANG Tao, MA Mei, WEN Hao. Study on BMP-2 on Inducing OPG^-/-BMSCs Differentiate into Osteogenesis in vitro[J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 186-190.

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Study on BMP-2 on Inducing OPG^-/-BMSCs Differentiate into Osteogenesis in vitro

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