Chronic Unpredictable Mild Stressed Rats Increased Their Movements in Open Field

doi:10.3969/j.issn.1674-5817.2012.03.001

Abstract

Abstract: Objective To explore the influence of chronic unpredictable mild stress(CUMS) on rats' open field behaviors. Methods Twenty-four SD rats were randomly assigned to three groups: A is control group, B is CUMS group, C is CUMS + citalopram group. Rats in group B and C were subjected to the CUMS schedule; Group A and B were submitted to administration with saline, while group C was pretreated with citalopram (CTP, 10 mg/kg, i.p.) every day. Body weight was measured every week. Sucrose preference and open field test were evaluated every three weeks. Animals were sacrificed after 6-week CUMS trial. Results CUMS rats, either with or without citalopram, weight gain was reduced compared to control (average 153.05 g, 127.69 g, 232.57 g, respectively). Rats in CUMS group had lower sucrose preference than which in control group and CUMS + citalopram group (average 60%, 73% and 71%, respectively). However, several behavioral indicators in open field, including total distance travelled, total mobile time and number of line crossings, were increased in CUMS treated rats,while which in control and CUMS + citalopram rats declined. Conclusion Chronic unpredictable mild stress may lead to rat's depression and elicit simultaneously some anxiety-like symptoms, such as increased motor activity.

Key words: Chronic unpredictable mild stress, Model of depression, Open field behavior, Rat, Citalopram

CLC Number:

Q95-33

LI Xia, WU Cheng-ye, XIAO Shi-fu, ZHENG Xian-jie, DONG Xiao-wei, PENG Su-fang, WANG Tao, XIAO Ze-ping. Chronic Unpredictable Mild Stressed Rats Increased Their Movements in Open Field[J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 175-181.

References

[1] Anisman H, Zacharko RM.Depression as a consequence of inadequate neurochemical adaptation in response to stressors[J]. Br J Psychiatry Suppl, 1992, (15):36-43.
[2] Solomon DA, Keller MB, Leon AC, et al.Multiple recurrences of major depressive disorder[J]. Am J Psychiatry, 2000, 157:229-233.
[3] Duman RS, Monteggia LM.A neurotrophic model for stress-related mood disorders[J]. Biol Psychiatry, 2006, 59(12):1116-1127.
[4] Stroud CB, Davila J, Moyer A.The relationship between stress and depression in first onsets versus recurrences: a meta-analytic review[J]. J Abnorm Psychol, 2008, 117(1): 206-213.
[5] Willner P, Towell A, Sampson D, et al.Reduction of sucrose preference by chronic unpredictable mild stress, and its restoration by a tricyclic antidepressant[J]. Psychopharmacology (Berl), 1987, 93(3):358-364.
[6] Willner P.Chronic mild stress (CMS) revisited: consistency and behavioural-neurobiological concordance in the effects of CMS[J]. Neuropsychobiology, 2005, 52(2):90-110.
[7] Katz RJ, Roth KA, Carroll BJ.Acute and chronic stress effects on open field activity in the rat: implications for a model of depression[J]. Neurosci Biobehav Rev, 1981, 5(2):247-251.
[8] Wang D, An SC, Zhang X.Prevention of chronic stress-induced depression-like behavior by inducible nitric oxide inhibitor[J]. Neurosci Lett, 2008, 433:59-64.
[9] Hu H, Su L, Xu YQ, et al.Behavioral and [F-18] fluorodeoxyglucose micro positron emission tomography imaging study in a rat chronic mild stress model of depression[J]. Neuroscience, 2010, 169(1):171-181.
[10] Gronli J, Murison R, Fiske E, et al.Effects of chronic mild stress on sexual behavior, locomotor activity and consumption of sucrose and saccharine solutions[J]. Physiol Behav, 2005, 84(4):571-577.
[11] Harris RB, Jun Zhou, Youngblood BD, et al.Failure to change exploration or saccharin preference in rats exposed to chronic mild stress[J]. Physiol Behav, 1998, 63(1):91-100.
[12] Matthews K, Forbes N, Reid IC.Sucrose consumption as an hedonic measure following chronic unpredictable mild stress[J]. Physiol Behav,1995, 57(2):241-248.
[13] Forbes NF, Stewart CA, Matthews K, et al.Chronic mild stress and sucrose consumption: validity as a model of depression[J]. Physiol Behav, 1996, 60(6):1481-1484.
[14] Hatcher JP, Bell DJ, Reed TJ, et al.Chronic mild stress-induced reductions in saccharin intake depend upon feeding status[J]. J Psychopharmacol, 1997, 11(4):331-338.
[15] D'Aquila PS, Newton J, Willner P. Diurnal variation in the effect of chronic mild stress on sucrose intake and preference[J]. Physiol Behav, 1997, 62(2):421-426.
[16] Phillips AG, Barr AM. Effects of chronic mild stress on motivation for sucrose: mixed messages[J]. Psychopharmacology (Berl), 1997, 134(4): 361-366; discussion 371-377.
[17] Harkin A, Houlihan DD, Kelly JP.Reduction in preference for saccharin by repeated unpredictable stress in mice and its prevention by imipramine[J]. J Psychopharmacol, 2002, 16(2):115-123.
[18] Pothion S, Bizot JC, Trovero F, et al.Strain differences in sucrose preference and in the consequences of unpredictable chronic mild stress[J]. Behav Brain Res, 2004, 155(1):135-146.
[19] Nirmal J, Babu CS, Harisudhan T, et al.Evaluation of behavioural and antioxidant activity of Cytisus scoparius Link in rats exposed to chronic unpredictable mild stress[J]. BMC Complement Altern Med, 2008, 8:15.
[20] Willner P.Validity, reliability and utility of the chronic mild stress model of depression: a 10-year review and evaluation[J]. Psychopharmacology (Berl), 1997, 134(4):319-329.
[21] Cuadra G, Zurita A, Gioino G, et al.Influence of different antidepressant drugs on the effect of chronic variable stress on restraint-induced dopamine release in frontal cortex[J]. Neuropsychopharmacology, 2001, 25(3):384-394.
[22] Ramanathan M, Kumar SN, Suresh B.Evaluation of cognitive function of fluoxetine, sertraline and tianeptine in isolation and chronic unpredictable mild stress-induced depressive Wistar rats[J]. Indian J Exp Biol, 2003, 41(11):1269-1272.
[23] Harro J, Haidkind R, Harro M, et al.Chronic mild unpredictable stress after noradrenergic denervation: attenuation of behavioural and biochemical effects of DSP-4 treatment[J]. Eur Neuropsychopharmacol, 1999, 10(1):5-16.
[24] Harro J, Tonissaar M, Eller M, et al.Chronic variable stress and partial 5-HT denervation by parachloroam-phetamine treatment in the rat: effects on behavior and monoamine neurochemistry[J]. Brain Res, 2001, 899(1-2):227-239.
[25] Mineur YS, Prasol DJ, Belzung C, et al.Agonistic behavior and unpredictable chronic mild stress in mice[J]. Behav Genet, 2003, 33(5):513-519.
[26] Lamers F, Oppen P, Comijs HC.Comorbidity patterns of anxiety and depressive disorders in a large cohort study: the Netherlands Study of Depression and Anxiety (NESDA)[J]. J Clin Psychiatry, 2011, 72(3):341-348.
[27] Browne CA, Clarke G, Dinan TG, et al.Differential stress-induced alterations in tryptophan hydroxylase activity and serotonin turnover in two inbred mouse strains[J]. Neuropharmacology, 2010, 60(4):683-691.
[28] Konkle A, Baker SL, Kentner AC, et al.Evaluation of the effects of chronic mild stressors on hedonic and physiological responses: sex and strain compared[J]. Brain Res, 2003, 992: 227-238.
[29] Beck KD, Luine VN.Sex differences in behavioral and neurochemical profiles after chronic stress: Role of housing conditions[J]. Physiol Behav, 2002, 75:661-673.
[30] Ma XC, Jiang D, Jiang WH, et al.Social isolation-induced aggression potentiates anxiety and depressive-like behavior in male mice subjected to unpredictable chronic mild stress[J]. PLoS One, 2011, 6(6): e20955.