Application of Synchrotron Radiation Microangiography on Rat Middle Cerebral Artery Occlusion Models

doi:10.3969/j.issn.1674-5817.2011.01.003

Abstract

Abstract: Objective To apply synchrotron radiation microangiography on the study of rat middle cerebral artery occlusion (MCAO) models. Method Twenty Sprague-Dawley rats weighing 300-350 g were divided into two groups: 4-0 suture group and 4-0 silicone-coated suture group. Immediately after MCAO surgery, synchrotron radiation angiography was conducted. Result The plain 4-0 nylon suture failed to completely occlude the middle cerebral artery (MCA) of the rat. There was still blood supply from anterior cerebral artery (ACA) and interior carotid artery (ICA). The silicone-coated 4-0 nylon suture successfully induced rat MCAO with 10% rats had fenestrated MCAs (2/20).The variants in these rats were occluded when the suture depth was 17 mm. Conclusion The silicone-coated 4-0 nylone suture successfully induced MCAO in rats weighing from 300 to 350 g, and the plain 4-0 suture was not able to induce complete ischemia in these rats. Synchrotron radiation microagiography is useful for studying rat MCAO models and evaluating rat cerebral abnormalities.

Key words: Angiography, Ischemic models, Rat, Synchrotron radiation

CLC Number:

Q95-33

YUAN Fa-lei, GUAN Yong-jing, XIAO Ti-qiao, XIE Hong-lan, YANG Guo-yuan, WANG Yong-ting. Application of Synchrotron Radiation Microangiography on Rat Middle Cerebral Artery Occlusion Models[J]. Laboratory Animal and Comparative Medicine, 2011, 31(1): 11-15.

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