Abstract: Objective To compare the changes of blood lipid, blood glucose, serum cytokine interleukin - 6 (IL 6) and interleukin 10 (IL-10), tumor necrosis factor - (TNF-α), a gamma interferon (IFN-γ) in mice with type 2 diabetes mellitus (T2DM) during early or late stage, and discuss the meanings of cellular immune function changes in T2DM mouse model. Methods Fourty-six male C57BL/6 mice at the age of 3 weeks were randomly divided into control group and model group (DM group). Animals in the model group were intraperitoneally injected with 50 mg streptozotocin(STZ)for 3 days and fed with high-fat diet. After 6 or 24 weeks injection, glucose tolerance, blood lipid (TG, CHOL, HDLC, LDLC) level and the contents of serum IL-6, IL-10, TNF-α, IFN-γ were measured and analyzed respectively. Results Six weeks after injection of STZ, abnormal glucose tolerance was observed in DM mice, with the significant higher level of lipid and serum IL-6, TNF-α, IFN-γ, IL-10 compared with normal control group (P<0.05 or P<0.01). With the development of disease process, the glucose and lipid metabolic disorder aggravated in DM group after 24 weeks of injection, and the levels of serum IL-6, TNF-α, IFN-γ, IL-10 were significantly higher than those of 6 weeks (P<0.01). Conclusion The STZ induced mouse model showed similar disease process in terms of blood glucose, blood lipids and cytokines changes in the different courses, which might be used as the potential disease animal model.

Key words: Diabetic Mice, Streptozotocin, Blood Sugar, Blood Lipid, Cytokine