Laboratory Animal and Comparative Medicine ›› 2023, Vol. 43 ›› Issue (1): 30-38.DOI: 10.12300/j.issn.1674-5817.2022.099

• Animal Model of Human Disease: Pharmacology • Previous Articles     Next Articles

Repair Effects of Xiaoyusan New Formula on Cartilage Injury and MMP-13 Expression in Knee Osteoarthritis Model Rabbits

Ruolin CUI1(), Qing WANG2, Ling YANG3, Wenchang FENG4, Zhiwei LIU1, Weiran TENG1, Bitao MA1, Leyang WANG1, Liping QIN1, Lilun JIN1()()   

  1. 1.Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Traditional Chinese Medicine Department, Shanghai 200092, China
    2.Huashan Hospital Affiliated to Fudan University Traditional Chinese Medicine Department, Shanghai 200040, China
    3.Department of Geriatrics, Fourth People's Hospital of Shanghai, Tongji University, Shanghai 200081, China
    4.Hubei Hospital of Integrated Traditional Chinese and Western Medicine Department of Education and Science, Wuhan 430015, China
  • Received:2022-07-04 Revised:2022-09-30 Online:2023-02-25 Published:2023-03-09
  • Contact: Lilun JIN


Objective To study the effect of Xiaoyusan new formula on the articular cartilage of knee osteoarthritis (KOA) rabbits and its mechanism. Methods A total of 42 New Zealand white rabbits aged 6 months were randomly divided into normal group, model group, ointment of Xiaoyusan group, and ointment of Xiaoyusan new formula group, with 10 rabbits in each group (the other 2 rabbits were used for model validation). Except for the normal group, the right knee joints of all rabbits in the other groups were prepared as KOA models according to the modified Hulth method. After 5 weeks of molding, the rabbits in ointment of Xiaoyusan group, ointment of Xiaoyusan New Formula group were given corresponding ointments for knee arthritis treatment, once a day, each time for 10 hours. After 2-week continuous administration and treatment, the knee joint cartilage of the four groups of rabbits was taken and the cartilage damage of each group was evaluated by Outerbridge grading method. The pathological changes of the cartilage, calcified layer and subchondral bone of the knee joint of rabbits in each group were observed by HE staining method under the light microscope, and the degree of cartilage degeneration was evaluated by Mankin's method. The expression of matrix metalloproteinase-13 (MMP-13) in the cartilage of rabbit knee joint in each group was deteced by immunohistochemistry. Results After the general observation of articular cartilage, the Outerbridge grading showed that the number of high-grade animals in ointment of Xiaoyusan group was reduced compared with the model group (P<0.05), and the number of high-grade animals in ointment of Xiaoyusan new formula group was also reduced (P<0.05) compared with ointment of Xiaoyusan group. HE staining showed that Mankin's scores of articular cartilage in the four groups ranked from high to low: model group (10.82±1.76), ointment of Xiaoyusan group (6.19±1.23), ointment of Xiaoyusan new formula group (2.64±1.18) and normal group (0.28±0.17). The difference among four groups was statistically significant (P<0.05). Immunohistochemical detection showed that the positive rates of MMP-13 expression in rabbit articular cartilage tissues in each group were (67.90±13.94)% of model group, (37.10±19.16)% of ointment of Xiaoyusan group, (13.60±3.10)% of ointment of Xiaoyusan new formula group and (3.20±2.39) % of normal group, ranking from high to low, and the difference among four groups was statistically significant (P<0.05). Conclusion Xiaoyusan new formula can repair articular cartilage degeneration in KOA rabbits and decrease the expression of MMP-13 in cartilage, which may be one of the mechanisms of the treatment.

Key words: Xiaoyusan new formula, Knee osteoarthritis, Articular cartilage, Matrix metalloproteinase-13

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