Laboratory Animal and Comparative Medicine ›› 2024, Vol. 44 ›› Issue (1): 52-61.DOI: 10.12300/j.issn.1674-5817.2023.094
• Animal Models of Human Diseases • Previous Articles Next Articles
Xi FANG1, Qingqing AO2, Chunhong LI1, Yiqiang OUYANG1, Songchao GUO1, Bing HU1()
Received:
2023-06-30
Revised:
2023-12-12
Online:
2024-02-25
Published:
2024-03-07
Contact:
Bing HU
CLC Number:
Xi FANG, Qingqing AO, Chunhong LI, Yiqiang OUYANG, Songchao GUO, Bing HU. Metabolomics Analysis of Tupaia belangeri Breast Tumor Model[J]. Laboratory Animal and Comparative Medicine, 2024, 44(1): 52-61.
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URL: https://www.slarc.org.cn/dwyx/EN/10.12300/j.issn.1674-5817.2023.094
分组 Group | 动物数量 n Number of Tupaia belangeri | 体重/g Body weight/g | 体长/cm Body length/cm | 肿瘤质量/g Tumor weight/g | 肿瘤体积/mm3 Tumor size/mm3 |
---|---|---|---|---|---|
DMBA造模未成瘤组 DMBA-induced group with breast cancer | 8 | 135.36±20.31 | 17.91±2.12 | 3.15±1.40 | 4 153.12±213.23 |
DMBA造模未成瘤组 DMBA-induced group without breast cancer | 12 | 131.11±11.54 | 17.68±1.32 | - | - |
正常对照组 Normal control | 12 | 123.65±10.54 | 17.73±0.65 | - | - |
Table 1 Basic information of Tupaia belangeri in three groups
分组 Group | 动物数量 n Number of Tupaia belangeri | 体重/g Body weight/g | 体长/cm Body length/cm | 肿瘤质量/g Tumor weight/g | 肿瘤体积/mm3 Tumor size/mm3 |
---|---|---|---|---|---|
DMBA造模未成瘤组 DMBA-induced group with breast cancer | 8 | 135.36±20.31 | 17.91±2.12 | 3.15±1.40 | 4 153.12±213.23 |
DMBA造模未成瘤组 DMBA-induced group without breast cancer | 12 | 131.11±11.54 | 17.68±1.32 | - | - |
正常对照组 Normal control | 12 | 123.65±10.54 | 17.73±0.65 | - | - |
Figure 1 Pathological results of breast cancer in Tupaia belangeriNote:A, Breast tumour tissue of the DMBA-induced breast cancer group; B, Breast tissue of the normal group. Scale bars, 50 μm.
Figure 2 Total ion flow maps of serum from each group of Tupaia belangeriNote: A, DMBA-induced breast cancer group (n=8); B, DMBA-induced without breast cancer group (n=12); C, Normal control group (n=12).
Figure 3 Score scatter plot of OPLS-DA model for each group of Tupaia belangeriNote:A, The OPLS-DA score plot of blood metabolites comparison between DMBA-induced breast cancer (DMBA-BC) group and normal control (NC) group; B, The OPLS-DA score plot of blood metabolites comparison between DMBA-induced with breast cancer (DMBA-BC) group and those without breast cancer (DMBA-nonBC); C, The OPLS-DA score plot of blood metabolites comparison between DMBA-induced without breast cancer (DMBA-nonBC) group and normal control (NC) group.
Figure 4 The volcano map (A-C) and heatmap of hierarchical clustering analysis (D-F) of differential metabolites between groups of Tupaia belangeriNote:A and D, DMBA-induced group with breast cancer (DMBA-BC) vs normal control (NC) group; B and E, DMBA-induced group with breast cancer (DMBA-BC) vs DMBA-induced group without breast cancer (DMBA-nonBC); C and F, DMBA-induced group without breast cancer (DMBA-nonBC) vs normal control (NC) group.
DMBA造模成瘤组vs正常对照组 DMBA-BC vs NC | DMBA造模成瘤组vsDMBA造模未成瘤组 DMBA-BC vs DMBA-nonBC | DMBA造模未成瘤组vs正常对照组 DMBA-nonBC vs NC | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
代谢物 Metabolites | VIP | P | Log-FC | CT | 代谢物 Metabolites | VIP | P | Log-FC | CT | 代谢物 Metabolites | VIP | P | Log-FC | CT |
甘油酸-3-磷酸 Glycerate-3-phosphate | 2.762 | <0.001 | -0.650 | ↑ | 十七烷酸 Margaric acid | 2.236 | 0.013 | 1.783 | ↑ | 甲氧基色胺 Methoxytry ptamine | 3.020 | <0.001 | 7.659 | ↑ |
谷氨酸 Glutamate | 2.086 | <0.001 | 3.051 | ↑ | 香芹酮 Carvone | 2.096 | 0.019 | 0.812 | ↑ | 肌苷 Inosine | 1.280 | <0.001 | 4.104 | ↑ |
腺苷 Adenosine | 2.561 | 0.001 | 2.995 | ↑ | 羟基脲 Hydroxyurea | 1.160 | 0.021 | 0.539 | ↑ | 葡萄糖庚糖 Glucose heptose | 1.303 | <0.001 | -1.476 | ↓ |
2,6-二磷酸果糖 2,6-Fructose diphosphate | 1.273 | 0.002 | 1.035 | ↑ | 1-磷酸鞘氨醇 Sphingosine 1- phosphate | 1.431 | 0.024 | 0.839 | ↑ | 甘油 Glycerol | 2.420 | <0.001 | -0.419 | ↓ |
4-羟基苯甲酸 4-Hydroxyben- zoic acid | 1.101 | 0.002 | 0.916 | ↑ | 1,5-脱水葡萄糖醇 1,5-Dehydrated glucose alcohol | 1.878 | 0.035 | 0.418 | ↑ | 月桂酸 Lauric acid | 2.291 | 0.001 | -1.011 | ↓ |
壬酸 Pelargonic acid | 3.167 | <0.001 | -1.640 | ↓ | 鸟氨酸 Ornithine | 1.344 | 0.036 | 1.042 | ↑ | 腺苷 Adenosine | 2.737 | 0.001 | 3.399 | ↑ |
1,5-脱水葡萄糖醇 1, 5-Anhydro- gluosol | 2.779 | <0.001 | -1.216 | ↓ | 丙醇二酸 Tartronic acid | 1.375 | 0.037 | 0.508 | ↑ | 氨基丁酸 Aminobutyric acid | 2.140 | 0.001 | 0.914 | ↑ |
α-酮戊二酸 α-Ketoglutaric acid | 2.382 | <0.001 | -1.771 | ↓ | 3-苯基乳酸 3-Phenyllactic acid | 1.248 | 0.038 | 1.373 | ↑ | 苏氨酸 Threonine | 1.738 | 0.004 | -0.702 | ↓ |
富马酸 Fumaric acid | 2.301 | 0.001 | -1.174 | ↓ | N-乙酰基-D-氨基胺 N-acetyl-D- aminoamine | 2.648 | 0 | -2.090 | ↓ | 1,2-脱水肌醇 1,2-Dehydrated Inositol | 2.925 | 0.005 | 18.130 | ↑ |
羟胺 Hydroxylamine | 1.492 | 0.001 | -0.975 | ↓ | α-酮异己酸 α-Ketoisocaproic acid | 2.371 | 0.007 | -0.891 | ↓ | 丝氨酸 Serine | 1.568 | 0.008 | -0.830 | ↓ |
2-羟基丁酸 2-Hydroxybutyric acid | 2.176 | 0.002 | -0.625 | ↓ | 己二酸 Adipic acid | 1.757 | 0.021 | -2.106 | ↓ | 吲哚乙酸 Indoleacetic acid | 2.373 | 0.010 | 14.098 | ↑ |
L-苹果酸 L-malic acid | 2.313 | 0.002 | -1.319 | ↓ | 柠檬酸 Citric acid | 1.043 | 0.021 | -0.742 | ↓ | 胸苷 Thymidine | 1.882 | 0.010 | 2.960 | ↑ |
邻苯二甲酸 Phthalate | 1.860 | 0.002 | -4.509 | ↓ | 邻苯二甲酸 Phthalic acid | 2.109 | 0.024 | -3.879 | ↓ | 柠檬酸 Citric acid | 2.049 | 0.010 | 3.048 | ↑ |
丙酮酸 Pyruvate | 2.085 | 0.008 | -1.193 | ↓ | 肌苷 Inosine | 1.690 | 0.037 | -1.451 | ↓ | 甘油酸-3-磷酸 Glycerate-3- phosphate | 1.664 | 0.015 | -0.574 | ↓ |
花生四烯酸 Arachidonic acid | 1.356 | 0.033 | -0.543 | ↓ | α-酮戊二酸 α-Ketoglutaric acid | 1.978 | 0.038 | -0.996 | ↓ | 谷氨酸 Glutamate | 1.292 | 0.016 | 2.622 | ↑ |
Table 2 Key differential metabolites between DMBA-induced group with breast cancer and control group of Tupaia belangeri
DMBA造模成瘤组vs正常对照组 DMBA-BC vs NC | DMBA造模成瘤组vsDMBA造模未成瘤组 DMBA-BC vs DMBA-nonBC | DMBA造模未成瘤组vs正常对照组 DMBA-nonBC vs NC | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
代谢物 Metabolites | VIP | P | Log-FC | CT | 代谢物 Metabolites | VIP | P | Log-FC | CT | 代谢物 Metabolites | VIP | P | Log-FC | CT |
甘油酸-3-磷酸 Glycerate-3-phosphate | 2.762 | <0.001 | -0.650 | ↑ | 十七烷酸 Margaric acid | 2.236 | 0.013 | 1.783 | ↑ | 甲氧基色胺 Methoxytry ptamine | 3.020 | <0.001 | 7.659 | ↑ |
谷氨酸 Glutamate | 2.086 | <0.001 | 3.051 | ↑ | 香芹酮 Carvone | 2.096 | 0.019 | 0.812 | ↑ | 肌苷 Inosine | 1.280 | <0.001 | 4.104 | ↑ |
腺苷 Adenosine | 2.561 | 0.001 | 2.995 | ↑ | 羟基脲 Hydroxyurea | 1.160 | 0.021 | 0.539 | ↑ | 葡萄糖庚糖 Glucose heptose | 1.303 | <0.001 | -1.476 | ↓ |
2,6-二磷酸果糖 2,6-Fructose diphosphate | 1.273 | 0.002 | 1.035 | ↑ | 1-磷酸鞘氨醇 Sphingosine 1- phosphate | 1.431 | 0.024 | 0.839 | ↑ | 甘油 Glycerol | 2.420 | <0.001 | -0.419 | ↓ |
4-羟基苯甲酸 4-Hydroxyben- zoic acid | 1.101 | 0.002 | 0.916 | ↑ | 1,5-脱水葡萄糖醇 1,5-Dehydrated glucose alcohol | 1.878 | 0.035 | 0.418 | ↑ | 月桂酸 Lauric acid | 2.291 | 0.001 | -1.011 | ↓ |
壬酸 Pelargonic acid | 3.167 | <0.001 | -1.640 | ↓ | 鸟氨酸 Ornithine | 1.344 | 0.036 | 1.042 | ↑ | 腺苷 Adenosine | 2.737 | 0.001 | 3.399 | ↑ |
1,5-脱水葡萄糖醇 1, 5-Anhydro- gluosol | 2.779 | <0.001 | -1.216 | ↓ | 丙醇二酸 Tartronic acid | 1.375 | 0.037 | 0.508 | ↑ | 氨基丁酸 Aminobutyric acid | 2.140 | 0.001 | 0.914 | ↑ |
α-酮戊二酸 α-Ketoglutaric acid | 2.382 | <0.001 | -1.771 | ↓ | 3-苯基乳酸 3-Phenyllactic acid | 1.248 | 0.038 | 1.373 | ↑ | 苏氨酸 Threonine | 1.738 | 0.004 | -0.702 | ↓ |
富马酸 Fumaric acid | 2.301 | 0.001 | -1.174 | ↓ | N-乙酰基-D-氨基胺 N-acetyl-D- aminoamine | 2.648 | 0 | -2.090 | ↓ | 1,2-脱水肌醇 1,2-Dehydrated Inositol | 2.925 | 0.005 | 18.130 | ↑ |
羟胺 Hydroxylamine | 1.492 | 0.001 | -0.975 | ↓ | α-酮异己酸 α-Ketoisocaproic acid | 2.371 | 0.007 | -0.891 | ↓ | 丝氨酸 Serine | 1.568 | 0.008 | -0.830 | ↓ |
2-羟基丁酸 2-Hydroxybutyric acid | 2.176 | 0.002 | -0.625 | ↓ | 己二酸 Adipic acid | 1.757 | 0.021 | -2.106 | ↓ | 吲哚乙酸 Indoleacetic acid | 2.373 | 0.010 | 14.098 | ↑ |
L-苹果酸 L-malic acid | 2.313 | 0.002 | -1.319 | ↓ | 柠檬酸 Citric acid | 1.043 | 0.021 | -0.742 | ↓ | 胸苷 Thymidine | 1.882 | 0.010 | 2.960 | ↑ |
邻苯二甲酸 Phthalate | 1.860 | 0.002 | -4.509 | ↓ | 邻苯二甲酸 Phthalic acid | 2.109 | 0.024 | -3.879 | ↓ | 柠檬酸 Citric acid | 2.049 | 0.010 | 3.048 | ↑ |
丙酮酸 Pyruvate | 2.085 | 0.008 | -1.193 | ↓ | 肌苷 Inosine | 1.690 | 0.037 | -1.451 | ↓ | 甘油酸-3-磷酸 Glycerate-3- phosphate | 1.664 | 0.015 | -0.574 | ↓ |
花生四烯酸 Arachidonic acid | 1.356 | 0.033 | -0.543 | ↓ | α-酮戊二酸 α-Ketoglutaric acid | 1.978 | 0.038 | -0.996 | ↓ | 谷氨酸 Glutamate | 1.292 | 0.016 | 2.622 | ↑ |
Figure 5 Analysis of different metabolic pathways among groups of Tupaia belangeriNote:A, Differential metabolic pathways between DMBA-induced group with breast cancer (DMBA-BC) and normal control (NC) group; B, Differential metabolic pathways between DMBA-induced (DMBA-BC) group with breast cancer and those without breast cancer (DMBA-nonBC); C, Differential metabolic pathways between DMBA-induced without breast cancer (DMBA-nonBC)group and normal control (NC) group.
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