Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology

doi:10.12300/j.issn.1674-5817.2021.019

Abstract

Abstract: Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology. Compared to PCR, it features ease of use, high efficiency, high sensitivity, high specificity, and does not need specific instruments, which allows it as an alternative to PCR and the most promising tool for rapid molecular diagnosis. RPA combined with lateral flow dipstick (LFD) (RPA-LFD) enables visual detection of amplified products and has promising applications for rapid nucleic acid detection of pathogens on site. The novel technique provides a new method for quality supervision and inspection of laboratory animals. In this paper, we reviewed the principle, research status, and technical difficulties of RPA-LFD, as well as the progress in rapid extraction of nucleic acid on site.

Key words: Recombinase polymerase amplification, Lateral flow dipstick, Rapid extraction of nucleic acid on site, Surveillance and control of animal epidemic diseases on site

CLC Number:

Q95-33
R-332

YU Lingzhi, TAO Lingyun, WEI Xiaofeng. Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology[J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 547-553.

Figures/Tables 1

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