Changes of C-reactive Protein and Inflammatory Factors in Juema Pig Model with High Altitude Multiple Organ Dysfunction Syndrome

doi:10.12300/j.issn.1674-5817.2020.119

Abstract

Abstract: Objective To investigate the changes of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1α) and C-reactive protein (CRP) in the development of inflammation in the Juma pig odel with high altitude multiple organ dysfunction syndrome (H-MODS). Methods Three doses (0.25, 0.35, and 0.50 mg/kg) of endotoxin lipopolysaccharide (LPS) were used to induce the H-MODS model (group B, C and D), and normal saline was used as control (group A). The serum levels of TNF-α, IL-1β and CRP were detected at different time points. Results The expression levels of TNF-α and IL-1β increased sharply after LPS infusion to the pigs, and reached the highest level at 6 h; the levels at 3-12 h of the pigs treated with LPS were increased compared with the level at 0 h of the pigs with the same treatment and the level of compared group A at the same time point (P < 0.05 or P < 0.01), and then decreased gradually. The expression levels of TNF-α at 24 h in groups B and D were significantly higher than that in the same group at 0 h and group A at the same time point (P < 0.05), and the expression level of TNF-α was significantly higher in group B than that in group A at 72 h (P < 0.05). The expression level of IL-1β at 24 h in group D was higher than that at 0 h and that in group A at the same time point (P < 0.05), and then decreased gradually. Compared with 0 h and group A, the expression levels of IL-1α in group D decreased significantly at 48 h and 72 h (P < 0.05 or P < 0.01). After intravenous infusion of LPS, the expression levels of CRP in groups B, C and D began to increase, and reached the maximum at 24-48 h; the expression levels of CRP in the other groups at 6-72 h were significantly higher than those in group A and at 0 h (P < 0.05 or P < 0.01). Conclusion Severe inflammatory reaction occurs during the development of H-MODS, and the expressions of inflammatory factors TNF-α and IL-1β increase rapidly first and then decrease sharply under immune regulation, displaying large fluctuations in short time until achieving an equilibrium; the CRP expression changes slowly throughout the inflammatory reaction in vivo.

Key words: High altitude multiple organ dysfunction syndrome, Juema pigs, Tumor necrosis factor-α, Interleukin-1β, C-reactive protein

CLC Number:

Q95-33
R-332

XIAO Pan, NIU Tingxian, LUO Xiaohong, WANG Hongyi, GUO Xiaoyu, LU Lu, FENG Xiaoming. Changes of C-reactive Protein and Inflammatory Factors in Juema Pig Model with High Altitude Multiple Organ Dysfunction Syndrome[J]. Laboratory Animal and Comparative Medicine, 2021, 41(3): 238-243.

Figures/Tables 2

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