Jejunal Nerve Regulation and Histomorphological Changes in Rats with Early Type 1 Diabetes

doi:10.12300/j.issn.1674-5817.2020.065

Abstract

Abstract: Objective To investigate functional changes of nitrergic and cholinergic nerves in the jejunum smooth muscle, and to observe jejunal histomorphology in rats with early type 1 diabetes. Methods Male Sprague Dawley (SD) rats were randomly divided into a normal control group and a diabetic model group. Rats in the diabetic model group were administered 60 mg/kg streptozotocin (STZ) by intraperitoneal injection. After 4 weeks, neurogenic contractile responses were induced by electric field stimulation, and functional changes in nitrergic and cholinergic nerves were observed in the isolated jejunum of diabetic rats. Histomorphological changes in the jejunum were observed by hematoxylin and eosin staining of paraffin-embedded tissue sections and immunohistochemistry. Results Compared with that in the normal rats, the jejunal intermuscular cholinergic nerve rats with early diabetes was damaged, whereas the nitrogenous nerve did not exhibit a such lesions. The villi of the jejunum were disrupted and disorderly, the muscularis was thickened, goblet cell numbers were decreased, and the ratio of villus length to intestinal crypt depth was diminished in diabetic rats. Conclusion In the early stage (4 weeks) of STZ-induced type 1 diabetes in rats, the neurological function and morphology of jejunal tissues were significantly changed. It suggests that the intestine is a sensitive organ in diabetes and has important clinical implications for the early prevention and detection of diabetes.

Key words: Diabetic rats, Jejunum, Nitrergic nerves, Cholinergic nerves, Histomorphology

CLC Number:

R-332
Q95-33

WANG Man, ZHI Minghua, GENG Xufang, TONG Miaomiao, LIANG Hongyu, ZHAO Ding. Jejunal Nerve Regulation and Histomorphological Changes in Rats with Early Type 1 Diabetes[J]. Laboratory Animal and Comparative Medicine, 2021, 41(2): 131-137.

Figures/Tables 4

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