乙酰唑胺与潘生丁对家兔腹主动脉扩张作用的比较

›› 2002, Vol. 22 ›› Issue (4): 213-217.

乙酰唑胺与潘生丁对家兔腹主动脉扩张作用的比较

复旦大学 1.附属中山医院核医学科；2.实验动物科学部,上海 200032

收稿日期:2002-03-27 出版日期:2002-01-25 发布日期:2013-03-19

Comparative Study on the Vasodilating Effect of Diamox and Dipyridamole on Abdominal Aorta in Rabbit

1.Department of Nuclear Medicine，Zhongshan Hospital, 2.Department of Experimental Animal Science, Fudan University, Shanghai 200032，China

Received:2002-03-27 Online:2002-01-25 Published:2013-03-19

摘要/Abstract

摘要： 为比较乙酰唑胺和潘生丁对家兔主动脉的扩张作用，并研究其扩张血管的机制，将6只家兔以戊巴比妥钠静脉麻醉后，截取腹主动脉环约5 mm，放入40ml以氧气饱和的Kreb's液中，中心穿线，一根将血管环固定，另一根线连接张力换能器，张力信号被传输到电脑中。给予略大于1.96×10^-2牛顿的张力，稳定10min，再将张力调至1.96×10^-2牛顿，观察张力稳定后记录血管张力变化。以重酒石酸去甲肾上腺素1mg收缩血管环，观察血管收缩后张力是否稳定。张力稳定后，分别加入乙酰唑胺15mg、潘生丁0.5mg，观察是否有扩血管作用。在观察到乙酰唑胺或潘生丁有扩血管作用的情况下，再于加入重酒石酸去甲肾上腺素1mg后15min时加入碘化钠0.045g，记录张力变化；并分别于加入重酒石酸去甲肾上腺素之前加入L-NAME、格列苯脲、吲哚美辛及消炎痛，观察以上几种药物对重酒石酸去甲肾上腺素的收缩血管作用及对乙酰唑胺和潘生丁的扩血管作用有无影响。结果：给予重酒石酸去甲肾上腺素1mg后，家兔主动脉环的张力上升（9.90±2.45）×10^-3牛顿（P＜0.05）。血管张力上升后在30min内保持稳定。乙酰唑胺能够明显对家兔主动脉环产生扩张作用，碘化钠对家兔主动脉环不能产生明显扩张作用，潘生丁对家兔腹主动脉环张力没有直接影响。克霉唑对乙酰唑胺所产生的血管扩张作用能够明显抑制，而硝基左旋精氨酸甲酯、优降糖、吲哚美辛对于乙酰唑胺的扩血管作用无影响。结论：乙酰唑胺能够明显扩张家兔主动脉，潘生丁对家兔腹主动脉没有直接的扩张作用。

关键词: 乙酰唑胺, 双嘧哒莫, 家兔, 血管扩张

Abstract: To investigate whether Diamox （Acetazolamide） or dipyridamole can dilate the abdominal aortic ring in rabbit directly and its mechanism also, six rabbits with the body weight of 2.33±0.18 kg were anaesthetized and the aortic rings about 5mm long were quickly excised and emerged in oxygen saturated Kreb’s solution. The aortic ring was connected to a force transducer by one thread and fixed by another. The tension signal was transmitted to and analyzed by a microcomputer. After 10 minutes of stabiliza-tion ,the preload was set at 1.96×10^-2 N and force signal was recoreded. Then the aor-tic ring was contracted with norepinephrine. Fifteen minutes later, 15 mg of Diamox or dipyridamole was given. If vasodilatation was observed, 0.045g of sodium iodide was given 15 minutes after norepinephrine. Besides this, N-omega-L-arginine-methyl-evSter, glibenclamide，clotrimazole or indomethacin was given before norepinephrine,and Diamox was given 15 minutes after norepinephrine. The results showed that norepinephrine induced rise in force did not change during the 30 minutes observation period. Diamox significantly dilated the aortic ring but sodium iodide did not. Dipyridamole did not influence the tension of aortic ring. Clotrimazole significantly abolished the vasodilating effect of Diamox. Nw-nitro-L-arginine-methy 1-ester, glibenclamide or indomethacin did not influence the vasodilating effect of Diamox. Conclusions：Diamox can directly dilate the rabbit’s aortic ring. Dipyridamole does not have any direct vasodilating effect on the rabbit’s abdominal aorta.

Key words: Acetazolamide, Dipyridamole, Rabbit, Vasodilatation

马宏星, 许兰文, 陈绍亮. 乙酰唑胺与潘生丁对家兔腹主动脉扩张作用的比较[J]. , 2002, 22(4): 213-217.

MA Hong-Xing, XU Lan-Wen, CHEN Shao-Liang. Comparative Study on the Vasodilating Effect of Diamox and Dipyridamole on Abdominal Aorta in Rabbit[J]. , 2002, 22(4): 213-217.

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