实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (3): 172-177.DOI: 10.3969/j.issn.1674-5817.2011.03.004

所属专题: 实验动物资源开发与利用

• 论著 • 上一篇    下一篇

转基因兔与克隆兔高效胚胎移植体系的建立

李海滨1, 田江天2, 宋军3, 赖良学3, 顾为望1   

  1. 1.南方医科大学实验动物中心暨比较医学研究所, 广州 510515;
    2.吉林大学畜牧兽医学院, 长春130062;
    3.中国科学院广州生物医药与健康研究院, 广州 510530
  • 收稿日期:2011-02-14 出版日期:2011-06-25 发布日期:2011-06-25
  • 作者简介:李海滨(1979-), 男, 硕士研究生, 研究方向: 实验动物学(转基因动物模型和胚胎工程), E-mail: jackie100@foxmail.com
  • 基金资助:
    国家科技部“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项课题(2009ZX10004-405)

Establishment of Efficient Embryo Transfer System in Transgenic Rabbit and Cloned Rabbit production

LI Hai-bin1, TIAN Jiang-tian2, SONG Jun3, LAI Liang-xue3, GU Wei-wang1   

  1. 1. Laboratory Animal Centre & Department of Comparative Medicine, Southern Medical University, Guangzhou 510515, China;
    2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;
    3. Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
  • Received:2011-02-14 Online:2011-06-25 Published:2011-06-25

摘要: 目的 建立高效的转基因兔和克隆兔的胚胎移植实验技术平台。方法 通过外科手术移植的方法将受精胚胎、转基因胚胎和体细胞核移植胚胎分别移植到同步发情或异步发情的受体兔输卵管。结果 移植受精胚胎、转基因胚胎和体细胞核移植胚胎的受体兔怀孕率分别为100%、66.67%、36.36%,产仔数分别为19、24及17只, 仔兔的成活率分别为90.48%、92.31%、17.65%。出生仔兔与移植胚胎数的比率分别为43.75%、21.31%、8.54%。结论 成功建立了高效的转基因兔和克隆兔的胚胎移植的实验技术平台。

关键词: 兔, 胚胎移植, 超数排卵, 受精卵胚胎, 转基因胚胎, 克隆胚胎

Abstract: Objective To establish an efficient platform of embryo transplantation for production of transgenic rabbits through virus transduction and somatic nuclear transfer approaches. Method Fertilized embryos, transgenic embryos, and nuclear transfer embryos were surgically transplanted into oviduct of synchronous or asynchronous estrus rabbits. Result After transplantation with fertilized embryos, transgenic embryos, and nuclear transfer embryos, the pregnant rates of surrogates were 100%, 66.67% and 36.36%, with the birth of 19, 24 and 17 pups. Survival rates of the pups delivered by recipients with fertilized embryos, transgenic embryos, and nuclear transfer embryos were 90.48%, 92.31% and 17.65%, meantime, the ratios of delivered pub to transferred embryos were 43.75%, 21.31% and 8.54%, respectively. Conclusion An efficient rabbit embryo transfer platform was successfully established, which would be the foundation of production of transgenic rabbits and cloned rabbits.

Key words: Rabbit, Embryo transplantation, Superovulation, Fertilized embryo, Transgenic embryo, Nuclear transfer embryo

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