实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (3): 222-226.DOI: 10.3969/j.issn.1674-5817.2015.03.009

所属专题: 实验动物资源开发与利用

• 论著 • 上一篇    下一篇

日本大耳白兔和新西兰兔体温周期变动规律的研究

王丰1, 郝飞1, 还红华1, 张元鹏1, 张波2, 邵国青1   

  1. 1.江苏省农业科学院兽医研究所, 农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心, 南京, 210014;
    2.金陵科技学院, 南京 211100
  • 收稿日期:2014-10-27 出版日期:2015-06-25 发布日期:2015-06-25
  • 作者简介:王 丰(1963-), 男, 硕士, 主要从事实验动物大数据处理研究。E-mail: jaaswang@163.com
  • 基金资助:
    2011年度江苏省工业和信息产业转型升级专项引导资金项目; “863”计划课题“动物精细养殖关键技术与装备”(2012AA101905)

Study on Changes of Body Temperature in Japanese White Rabbit and New Zealand Rabbit under Physiological Conditions

WANG Feng1, HAO Fei1, HUAN Hong-hua1, ZHANG Yuan-peng1, ZHANG Bo2, SHAO Guo-qing1   

  1. 1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences·Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture ·National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China;
    2. Jinling Institute of Technology, Nanjing 211100, China
  • Received:2014-10-27 Online:2015-06-25 Published:2015-06-25

摘要: 目的 探讨生理状况下普通级日本大耳白兔和新西兰兔的体温变化规律。方法 在23.5~28℃、相对湿度54%~65%的环境下,利用稻盛弘TM无线自动测温平台对处于自由状态下的家兔进行自动连续测温。对31.2万次形成12 480个家兔时点体温数据分析。结果 早晚定时定量饲喂条件下,参试动物昼夜体温呈W型规律性波动,1∶00~6∶00、15∶00~18∶00为体温上升期,7∶00~11∶00、20∶00~0∶00为体温下降期,5∶00~8∶00、18∶00~20∶00为体温波峰期,23∶00~2∶00、11∶00~15∶00为体温波谷期。体温波动较大的区间集中在采食后5 h以内,4∶00~7∶00、8∶00~11∶00、16∶00~24∶00的14 h内每4 h体温变化较大。0∶00~4∶00、7∶00~8:00、11:00~16:00的10 h内体温变化较小,适合进行热原实验。结论 家兔体温在采食后2~5 h呈体温下降趋势,23∶00~2∶00、11∶00~15∶00为体温波谷期。热原检查试验适合在0∶00~3∶00和12∶00~15∶00进行。目前生物制品所涉及动物实验中被广泛采用的按24 h平均时间间隔测温的方法或有商榷余地。

关键词: 大数据, 日本大耳白兔, 新西兰兔, 体温周期变化规律, 热原实验

Abstract: Objective To investigate the change rules of the body temperature in conventional Japanese white rabbits and New Zealand rabbits under physiological condition. Methods In the environment of 23.5-28 ℃ and relative humidity 54%-65%, the body temperature of rabbits in free stateus were determined continuously by using TOSHONG wireless automatic temperature measurement platform. The 12 480 points of rabbits’ temperature picked from 312 000 times of measurements were analysed. Results Feeding the rabbits quantitatively in the same time in morning and evening, the tested animals’ circadian body temperature is the W type. 1∶00-6∶00, 15∶00-18∶00 are the rise period of temperature, 7∶00-11∶00, 20∶00-0∶00 are the descend period of temperature, 5∶00-8∶00, 18∶00-20∶00 are the peak of temperature, 23∶00-2∶00, 11∶00-15∶00 are the trough of temperature. The rabbits’ body temperature changes bigger than usual in the next 5 hours after they eating. Conclusions The rabbits’ body temperature showed downtrend in the next 2-5 hours after they eating, 23∶00-2∶00, 11∶00-15∶00 are the trough of temperature. Pyrogen test is suitable for to taken in 0∶00-3∶00 and 12∶00-15∶00. The method which widely used in animal experiments according to the method of average 24 hours time interval measurement may be remained consultative.

Key words: Big data, Japanese white rabbit, New Zealand rabbit, Rule of body temperature period, Pyrogen test

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