关键词: 垫料, 细胞毒性, 生长活性

Abstract: Objective To investigate the cytotoxic al effects of beddings on growth activity of mouse cell lines. Methods The acetone extraction of three kind beddings(com cob, pine wood shaving, poplar wood shaving ) were respectively added in mouse hepatoma cell line Hepa 1 -6(Hepa 1 -6), the Lewis Lung carcinoma (LLC)and the Melanoma B16(B16) with different concentration. MTT method was used to detect the cell grown activity and compare the cytotoxical effects of different beddings(pine wood shaving, poplar wood shaving and corn cob). Results The acetone extraction of three kinds of bedding provided with inhibition effects on three cell lines. The coefficients on Hepal-6 in linear regression models are: corn cob -0.875，pine wood shaving -0.747，poplar wood shaving -0.826. The coefficients oil LLC are: com cob -0.527，pine wood shaving -0.812, poplar wood shaving -0.865. The coefficients on B16 are: corn cob -0.727，pine wood shaving -0.860，poplar wood shaving -0.888. The 50% concentration of inhibition (IC50) of beddings to Hepal-6 are: com cob 8.86 mg/ml, pine wood shaving 19.10 mg/ml, poplar wood shaving 91.37mg/ml. The IC50 of beddings to LLC are: corn cob 4.42 mg/ml, pine wood shaving 7.72 mg/ml, poplar wood shaving 24.5 mg/ml. The IC50 of beddings to B16 are: corn cob 8.86 mg/ml, pine wood shaving 19.10 mg/ml, poplar wood shaving 91.37 mg/ml. Conclusion There are evident negative relativity between the three beddings and Hepal-6、LLC、B16 cell grown activity (p<0.001). Cytotoxic role in cell growth inhibition is pine wood shaving＞poplar wood shaving＞ corn cob.

Key words: Beddings, Cytotoxicity, Cell growth activity