三种垫料丙酮提取物对小鼠肿瘤细胞株毒性作用观察

›› 2009, Vol. 29 ›› Issue (4): 244-247.

三种垫料丙酮提取物对小鼠肿瘤细胞株毒性作用观察

中国医学科学院北京协和医学院实验动物所，北京，100021

收稿日期:2009-03-10 出版日期:2009-04-30 发布日期:2009-04-30

The Cytotoxic Effects of Acetone Extractions from Three Kind Beddings on Mouse Tumour Cell Lines

Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS)& Peiking Union Medical College (PUMC), Beijing, 100021, China

Received:2009-03-10 Online:2009-04-30 Published:2009-04-30

摘要/Abstract

摘要： 目的探讨实验动物垫料对小鼠细胞生长活性的影响及其可能的细胞毒性。方法体外培养的小鼠肝癌细胞Hepa1-6（mouse hepatoma cell line Hepa1-6，Hepa1-6）、小鼠肺癌细胞（LewisLung carcinoma，LLC）和小鼠黑色素瘤细胞B16（Melanoma，B16）培养液中分别加入不同浓度的3种实验动物常用垫料（玉米芯、松木、杨木）丙酮提取物，采用MTT方法测定细胞生长活性，并比较3种垫料对细胞的生长毒性。结果小鼠Hepa1-6细胞生长活性与3种垫料之间的线性相关系数分别为：玉米芯-0.875，松木-0.747，杨木-0.826；LLC细胞分别为：玉米芯-0.527，松木-0.812，杨木-0.865；B16细胞分别为：玉米芯-0.727，松木-0.860，杨木-0.888.松木、杨木、玉米芯垫料对小鼠Hepa1-6细胞的半致死量（IC50）分别为：8.86，19.10，91.37 mg/ml；对LLC细胞的IC50分别为：4.42，7.72，24.65 mg/ml；对B16细胞的ICso分别为：8.86，19.10，91.37 mg/ml.结论 3种垫料与小鼠细胞Hepa1-6、LLC、B16的生长活性之间具有显著负相关性（P＜0.001），其抑制细胞生长作用依次为：松木>杨木>玉米芯。

关键词: 垫料, 细胞毒性, 生长活性

Abstract: Objective To investigate the cytotoxic al effects of beddings on growth activity of mouse cell lines. Methods The acetone extraction of three kind beddings(com cob, pine wood shaving, poplar wood shaving ) were respectively added in mouse hepatoma cell line Hepa 1 -6(Hepa 1 -6), the Lewis Lung carcinoma (LLC)and the Melanoma B16(B16) with different concentration. MTT method was used to detect the cell grown activity and compare the cytotoxical effects of different beddings(pine wood shaving, poplar wood shaving and corn cob). Results The acetone extraction of three kinds of bedding provided with inhibition effects on three cell lines. The coefficients on Hepal-6 in linear regression models are: corn cob -0.875，pine wood shaving -0.747，poplar wood shaving -0.826. The coefficients oil LLC are: com cob -0.527，pine wood shaving -0.812, poplar wood shaving -0.865. The coefficients on B16 are: corn cob -0.727，pine wood shaving -0.860，poplar wood shaving -0.888. The 50% concentration of inhibition (IC50) of beddings to Hepal-6 are: com cob 8.86 mg/ml, pine wood shaving 19.10 mg/ml, poplar wood shaving 91.37mg/ml. The IC50 of beddings to LLC are: corn cob 4.42 mg/ml, pine wood shaving 7.72 mg/ml, poplar wood shaving 24.5 mg/ml. The IC50 of beddings to B16 are: corn cob 8.86 mg/ml, pine wood shaving 19.10 mg/ml, poplar wood shaving 91.37 mg/ml. Conclusion There are evident negative relativity between the three beddings and Hepal-6、LLC、B16 cell grown activity (p<0.001). Cytotoxic role in cell growth inhibition is pine wood shaving＞poplar wood shaving＞ corn cob.

Key words: Beddings, Cytotoxicity, Cell growth activity

孟金萍，孙淑华，杨旭，王艳蓉，刘云波. 三种垫料丙酮提取物对小鼠肿瘤细胞株毒性作用观察[J]. , 2009, 29(4): 244-247.

MENG Jin-ping，SUN Shu-hua，YANG Xu，WANG Yan-rong，LIU Yun-bo. The Cytotoxic Effects of Acetone Extractions from Three Kind Beddings on Mouse Tumour Cell Lines[J]. , 2009, 29(4): 244-247.

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