IL-2基因修饰的树突状细胞诱导抗胰腺癌免疫效应的实验研究

›› 2009, Vol. 29 ›› Issue (4): 241-243.

IL-2基因修饰的树突状细胞诱导抗胰腺癌免疫效应的实验研究

1.复旦大学实验动物科学部，上海，200032；2.中科院上海实验动物中心，上海，201615；3.上海市华东医院普外科，上海，200040

收稿日期:2008-05-30 出版日期:2009-04-30 发布日期:2009-04-30

Induction of T Lymphocytes by Dendritic Cells Transfected with IL-2 Gone for Therapy of Nude Mice Transplanted with Pancreas Cancer Cell Line PC3

1. Department of Laboratory Animal Science, FuDan University, Shanghai 200032, China;2. Shanghai Laboratory Animal Center, The Chinese Academy of Sciences, Shanghai 201615，China; 3. Department of Surgery, Hua Dong Hospital, Shanghai 200040, China)

Received:2008-05-30 Online:2009-04-30 Published:2009-04-30

摘要/Abstract

摘要： 目的将白细胞介素2（IL-2）基因修饰树突状细胞（DC），观察其和对胰腺癌荷瘤裸鼠的治疗作用。方法将IL-2基因转染单个核细胞来源的DC后，用于激发自体细胞毒性T淋巴细胞（CTL），检测其对CTL的增殖和人胰腺癌PC3细胞杀伤性的影响；检测CTL对荷人胰腺癌PC3细胞肿瘤裸鼠肿瘤生长情况影响和对肿瘤细胞分裂指数和端粒酶活性的影响。结果 IL-2基因修饰的DC细胞所激发的CTL对胰腺癌PC-3细胞有较强的抑制作用，并能抑制荷瘤裸鼠肿瘤细胞的分裂指数和端粒酶活性。结论 IL-2基因修饰DC细胞激活的CTL抗PC-3肿瘤免疫作用明显增强，能明显抑制人胰腺癌PC3细胞种植瘤的生长。

关键词: 树突状细胞, 白细胞介素2, 细胞毒性T淋巴细胞, 胰腺肿瘤, 细胞株

Abstract: Objective To study the antitumor effect of cytotoxic T lymphocytes (CTL) induced by dendritic cells (DCs) transfected with IL-2 gene. Methods DCs collected from peripheral blood monocytes were transfected with IL-2 gene and CTL was induced by it. The proliferation of the CTL and its cytotoxic activity to PC3 was assessed. Nude mice with PC3 transplanted tumor were treated with CTL. The kinetics growth was recorded，RT-PCR-ELISA was used to determine the telomerase of transplanted tumor. Result CTL induced by DCs transfected with IL-2 gene can kill PC3 effectively, and it suppress the growth of experimental PC3 tumor and its telomerase. Conclusion DCs transfected with IL-2 gene activate the anti-tumor immune effect of CTL T lymphocytes and it may be a good immune therapy to malignant tumor.

Key words: Dendritic Cells, IL-2, Cytotoxic T Lymphocytes, Pancreatic neoplasms, Cell line

乔伟伟1，顾坚忠2，杜卫东3. IL-2基因修饰的树突状细胞诱导抗胰腺癌免疫效应的实验研究[J]. , 2009, 29(4): 241-243.

QIAO Wei-wei1，GU Jian-zhong2，DU Wei-dong3. Induction of T Lymphocytes by Dendritic Cells Transfected with IL-2 Gone for Therapy of Nude Mice Transplanted with Pancreas Cancer Cell Line PC3[J]. , 2009, 29(4): 241-243.

[1]	阮蕾颖, 孙晓梅. 小鼠树突状细胞亚群分类及其比较研究进展[J]. 实验动物与比较医学, 2021, 41(2): 174-180.
[2]	顾云浩, 曹晨洁, 胡碧原, 王俊, 韩东冬, 许爱华. PFC体内递增诱导建立S180肿瘤多药耐药模型及其稳定性观察[J]. 实验动物与比较医学, 2015, 35(5): 367-373.
[3]	程冬冬, 刘蕾, 张智长, 杨庆诚. 组蛋白去乙酰化酶抑制剂抗骨肉瘤活性的体内实验研究[J]. 实验动物与比较医学, 2013, 33(1): 47-51.
[4]	屠珏, 徐剑钦, 潘永明, 刘志华, 孔勤, 陈民利. WHBE兔骨髓、外周血和脾脏来源树突状细胞的比较[J]. 实验动物与比较医学, 2012, 32(3): 209-214.
[5]	姚明, 李静, 荚德水, 张方琳, 闫明霞, 刘蕾, 张书霞. 人肺癌高转移细胞系的转移相关基因观察[J]. 实验动物与比较医学, 2011, 31(2): 79-82.
[6]	周谦君1，2，范慧敏1，刘中民1. IL-4,GM-CSF体外诱导猪单核来源树突状细胞和功能鉴定[J]. , 2010, 30(3): 174-179.
[7]	洑翔科1, 邓忠彬1, 朱伟2, 周正宇1, 王禹斌1, 周慧英1, 薛智谋1. 人多发性骨髓瘤荷瘤SCID小鼠模型的建立及其生长特性[J]. , 2004, 24(4): 230-233.
[8]	吴淑燕1，古涛2，周桓2，王响英1，李苏安1，毛棣华1，朱一蓓2，陈成2，周时勇2，张学光2. 小鼠骨髓前体细胞来源的树突状细胞超微结构[J]. , 2004, 24(1): 34-36.
[9]	张宜俊, 刘光泽, 王洪敏, 吕丽春, 章谷生. HBsAg特异性CTL诱发HBV转基因小鼠肝损伤的实验研究[J]. , 1999, 19(1): 8-10.
[10]	王建飞，王晓明，邵伟娟. 小鼠肝炎病毒(MHV-1)单克隆抗体杂交瘤细胞株的建立和鉴定[J]. , 1993, 13(1): 8-11.