鼠痘病毒潜伏感染的研究

›› 2000, Vol. 20 ›› Issue (2): 82-85.

鼠痘病毒潜伏感染的研究

1.中科院细胞研究所动物实验室，上海 200031；2.上海生物制品研究所，上海 200052

收稿日期:1999-12-20 出版日期:2000-01-25 发布日期:2013-03-19

Study on Latent Infection of Mousepox

1. Department of Experiment Animal, Institute of Cell Biology，Chinese Academy of Science, Shanghai 200031,China；2.Shanghai Institute of Biological Products,Shanghai 200052,China

Received:1999-12-20 Online:2000-01-25 Published:2013-03-19

摘要/Abstract

摘要： 对经实验前后的普通级和清洁级KM，BALB/c，C57BL小鼠，分别用IFA、ELISA、PAP和ABC法方法检测小鼠血清中的LCMV、EHFV、MPV、MHV，仙台病毒抗体和抗原；电镜检查组织中的病毒颗粒。结果显示：未经实验及经实验15d和30d后的各品系小鼠血清病毒抗体均为阴性；经实验60d后检测普通级KM和BALB/c小鼠鼠痘病毒抗体，阳性比例分别为5/30和6/30。病毒抗体检测血清抗体阴性，经实验后的普通级与清洁级KM，BALB/c小鼠小肠粘膜上皮细胞胞浆内、肝细胞及枯胚氏细胞胞浆内、脾脏的巨噬细胞胞浆内和树突细胞胞浆内、肠系漠淋巴结，小肠粘膜下集合淋巴结的网状细胞，巨噬细胞经PAP，ABC染色为阳性；电镜发现病毒颗粒，胞浆内出现内质网扩展、线粒体嵴肿胀、胞浆内空泡增多、高尔基复合体变形等病理学变化。

关键词: 鼠痘, 潜伏感染

Abstract: The conventional and clean KM，BALB/c and C57BL/6J mice were detected for LCM（ lymphocytic choriomeningitis virus）, EHFV（ epidemic hemorrhagic fever virus），Mouse pox, MPV（ mouse hepatitis virus）, Sendai virus infetion by using IFA，ELISA,ABC（ avidin-biotin-peroxidse complex）, and electron microscope before and 15,30，60 days after undergoing animal experiments.The results revealed that 60 days after the animal experiments, ectromelia virus was found in the liver，mesenteric lym-phaden，Payer’s patches, and spleen of seronegative（ELISA，IFA ） KM and BALB/c mice，while in the other groups no ectromelia virus infection were found.The findings suggest that the mice with latent infection of ectromelia virus,despite of sero-negative,would give rise to an outbreak when they were under a long stress of animal experiment ,and the viral antigen detection of Mousepox should be used in routine microbio-logical monitoring.

Key words: Mousepox, Latent infection

李嘉荣1, 钱琴2, 来永禄2, 金风2, 宋新英2. 鼠痘病毒潜伏感染的研究[J]. , 2000, 20(2): 82-85.

LI Jia-Rong-1, QIAN Qin-2, LAI Yong-Lu-2, JIN Feng-2, SONG Xin-Ying-2. Study on Latent Infection of Mousepox[J]. , 2000, 20(2): 82-85.

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