不同周龄段雄性长爪沙鼠肾小体的形态变化

doi:10.12300/j.issn.1674-5817.2024.011

实验动物与比较医学 ›› 2024, Vol. 44 ›› Issue (5): 487-494.DOI: 10.12300/j.issn.1674-5817.2024.011

• 实验动物资源开发与利用 • 上一篇下一篇

不同周龄段雄性长爪沙鼠肾小体的形态变化

李梦¹(), 陈柏安¹^,²^,³, 卢静¹^,²^,³()

^1.首都医科大学实验动物部, 北京 100069
^2.首都医科大学基础医学院, 北京 100069
^3.首都医科大学神经再生修复研究北京市重点实验室, 北京 100069

收稿日期:2024-01-16 修回日期:2024-06-03 出版日期:2024-11-06 发布日期:2024-10-25
通讯作者: 卢静（1969—），女，博士，教授，主要从事学习记忆功能障碍疾病动物模型和实验动物资源研究。E-mail： lujing@ccmu.edu.cn
作者简介:李梦（1981—），男，硕士，主管技师，研究方向：长爪沙鼠病理。E-mail: lm_bshlm@126.com
基金资助:
国家重点研发计划重点专项子课题“新型实验动物资源库服务科技创新能力提升的关键技术研究与示范”(2021YFF0702805)

Morphological Changes of Renal Corpuscles in Male Mongolian Gerbils at Different Ages

LI Meng¹(), CHEN Bai'an¹^,²^,³, LU Jing¹^,²^,³()

^1.Department of Experimental Animals, Capital Medical University, Beijing 100069, China
^2.School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
^3.Beijing Municipal Key Laboratory for Neural Regeneration and Repairing, Capital Medical University, Beijing 100069, China

Received:2024-01-16 Revised:2024-06-03 Published:2024-10-25 Online:2024-11-06
Contact: LU Jing, E-mail: lujing@ccmu.edu.cn

摘要/Abstract

摘要：

目的通过观察比较不同周龄段的雄性长爪沙鼠的肾脏在最大纵切面上肾小体的变化，初步探索不同周龄段雄性长爪沙鼠肾小体可能存在的变化规律，为选择雄性长爪沙鼠用于肾病相关致病机制及药物筛选等研究提供基础数据支持。方法分别取12周龄、48周龄、72周龄3个周龄段的雄性长爪沙鼠肾脏，从最大冠状面纵切后制成肾脏组织切片；苏木精-伊红染色后，全景扫描成电子组织切片，利用CaseViewer拍摄最大纵切面上肾小体图片，并测量肾小体及其所包含的肾小球、肾小囊面积；使用IBM SPSS Statistics 27进行统计分析，使用GraphPad Prism 8制作图表。结果与12周龄相比，48周龄时肾小体（P=0.029）、肾小球（P=0.001）的面积均显著增大，肾小囊面积没有明显变化（P=0.478），肾小球在其肾小体中的面积占比有增大趋势，但差异无统计学意义（P=0.163）；与48周龄相比，72周龄时肾小体、肾小球和肾小囊面积均显著增大（P<0.001），但肾小球在其肾小体中的面积占比显著减小（P<0.001）。结论 12～72周龄的雄性长爪沙鼠肾小体持续增大，在这个增大过程中可能存在一定规律，即肾小球与肾小体的增大趋势相对一致；而肾小囊与肾小体相比，其增大趋势存在一定滞后性，推测肾小体增大主要是由肾小球增大引起，进而导致肾小囊被动增大造成的；肾小体增大可能是通过多次“肾小球增大–引发肾小囊增大”完成。

关键词: 雄性长爪沙鼠, 肾小体, 肾小球, 肾小囊

Abstract:

Objective By observing and comparing the changes in renal corpuscles at the largest longitudinal section in male Mongolian gerbils of different ages, this study preliminarily explored potential patterns of renal corpuscle changes, providing foundational data for the selection of male Mongolian gerbils for research on the pathogenic mechanisms and drug screening of nephropathy. Methods Kidney samples were collected from male Mongolian gerbils aged 12, 48, and 72 weeks. After making longitudinal cuts along the largest coronal plane in the middle, kidney tissue sections were prepared. Following HE staining, panoramic electronic tissue sections were scanned, and images of renal corpuscles at the largest longitudinal plane were captured using CaseViewer. The areas of renal corpuscles and their glomeruli and renal vesicles were measured. Statistical analysis was performed using IBM SPSS Statistics 27, and figures were created using GraphPad Prism 8. Results Compared with the 12-week group, both the area of renal corpuscle (P=0.029) and glomerulus (P=0.001) significantly increased in the 48-week group, while there was no significant change in the area of renal vesicle (P=0.478). The proportion of glomerular area within the renal corpuscle showed an increasing trend but the difference was not statistically significant (P=0.163). Compared to the 48-week group, the areas of renal corpuscle, glomerulus, and renal vesicle were all significantly larger in the 72-week group (P<0.001), but the proportion of glomerular area within renal corpuscle decreased significantly (P<0.001). Conclusion The renal corpuscles in male Mongolian gerbils continued to increase from 12 to 72 weeks of age. There might be a certain pattern in this process of enlargement, where the trends of glomerular and renal corpuscle enlargement were relatively consistent. However, the enlargement of renal vesicles appeared to lag behind that of the renal corpuscle. It was speculated that the enlargement of renal corpuscles was mainly caused by the passive enlargement of renal vesicles due to glomerular enlargement. The enlargement of renal corpuscles might be achieved through multiple cycles of "glomerular enlargement - triggering renal vesicle enlargement".

Key words: Male Mongolian gerbil, Renal corpuscles, Glomerulus, Renal vesicle

中图分类号:

Q95-33

李梦,陈柏安,卢静. 不同周龄段雄性长爪沙鼠肾小体的形态变化[J]. 实验动物与比较医学, 2024, 44(5): 487-494. DOI: 10.12300/j.issn.1674-5817.2024.011.

LI Meng,CHEN Bai'an,LU Jing. Morphological Changes of Renal Corpuscles in Male Mongolian Gerbils at Different Ages[J]. Laboratory Animal and Comparative Medicine, 2024, 44(5): 487-494. DOI: 10.12300/j.issn.1674-5817.2024.011.

图/表 5

图1 雄性长爪沙鼠不同面积肾小体的苏木精-伊红染色结果注：40×10倍焦距下不同横截面积（A～H图分别为2 476.0、3 211.0、5 089.6、6 502.2、8 534.3、10 320.7、13 010.6、15 427.0 μm2）的肾小体图片（红色箭头所指），比例尺大小为20 μm。

Figure 1 Hematoxylin and eosin staining results of renal corpuscles of different sizes in male Mongolian gerbilsNote： Images of renal corpuscles with different cross-sectional areas (the area of A-H images are 2 476.0, 3 211.0,, 5 089.6, 6 502.2, 8 534.3, 10 320.7, 13 010.6, 15 427.0, μm 2, respectively) at a magnification of 40 × 10 times (indicated by red arrows), with a scale bar size of 20 μm.

图2 不同周龄雄性长爪沙鼠的肾脏最大纵切面上肾小体、肾小球、肾小囊大小比较注：每只雄性长爪沙鼠随机选取30个视野，测量30套肾小体、肾小球和肾小囊的面积（n=6）；A～C是3个周龄段肾小体、肾小球、肾小囊面积散点图；D～F是3个周龄段肾小体、肾小球、肾小囊面积趋势图。

Figure 2 Comparison of sizes of renal corpuscles, glomeruli, and renal vesicles on the largest longitudinal section of the kidneys in male Mongolian gerbils of different agesNote：30 fields of view were randomly selected from each male Mongolian gerbil, and the areas of 30 sets of renal corpuscles, glomeruli, and renal vesicles were measured (n=6) ; A-C are scatter plots of renal corpuscle, glomerulus, and renal vesicle area at three age groups; D-F are trend charts of renal corpuscle, glomerulus, and renal vesicle area at three age groups.

图3 不同周龄组随机抽取1只雄性长爪沙鼠的肾脏最大纵切面上肾小体、肾小球、肾小囊大小比较注：不同周龄组随机选取1只雄性长爪沙鼠，测量其肾脏最大纵切面上所有肾小体、肾小球和肾小囊的面积，12周测量了236套肾小体、肾小球、肾小囊，48周测量了302套，72周测量了298套。A～C是肾小体、肾小球、肾小囊面积散点图；D～F是肾小体、肾小球、肾小囊面积趋势图。

Figure 3 Comparison of the size of renal corpuscles, glomeruli, and renal vesicles on the largest longitudinal section in a male Mongolian gerbil randomly selected from different age groupsNote： Randomly select one male Mongolian gerbil at each week of age and measure the area of all renal corpuscles, glomeruli, and renal vesicles on the largest longitudinal section of its kidney；236, 302 and 298 sets of renal corpuscles, glomeruli, and renal vesicles were measured at 12 weeks, 48 weeks, and 72 weeks respectively; A-C are scatter plots of renal corpuscle, glomerulus, and renal vesicle area; D-F are trend charts of renal corpuscle, glomerulus, and renal vesicle area.

图4 雄性长爪沙鼠不同大小肾小球占比的苏木精-伊红染色结果注：40×10倍焦距下不同肾小球占比 [A：54.1%；B：66.9%（左下）/69.9%（右上）；C：73.7%；D：80.9%；E：83.0%；F：97.0%] 的肾小体图片（红色箭头所指），比例尺大小为20 μm。

Figure 4 Hematoxylin and eosin staining results of the different proportion of glomerular in male Mongolian gerbilsNote： Images of renal corpuscles with different proportions of glomeruli at magnification of 40 × 10 times (indicated by red arrows). A: 54.1%; B: 66.9% (bottom left)/69.9% (top right); C: 73.7%; D: 80.9%; E: 83.0%; F: 97.0%, with a scale bar size of 20 μm.

图5 不同周龄雄性长爪沙鼠肾脏最大纵切面上肾小球占比大小比较注：A，C为肾小球占比散点图；B，D为肾小球占比趋势图。A～B，每只雄性长爪沙鼠随机选取30个视野，测量30套肾小体、肾小球和肾小囊的面积（n=6）；C～D，每个周龄随机取1只雄性长爪沙鼠，测量肾脏最大纵切面上所有肾小体、肾小球、肾小囊的面积，12周龄测量了236套肾小体、肾小球、肾小囊，48周龄测量了302套，72周龄测量了298套，计算肾小球占肾小体的面积之比。

Figure 5 Comparison of the proportion of glomerular area on the largest longitudinal section of the kidneys in male Mongolian gerbils of different agesNote： A and C are scatter plots of glomerular proportions; B and D are the trend charts of glomerular proportion. A-B 30 fields of view were randomly selected from each male Mongolian gerbil, and the areas of 30 sets of renal corpuscles, glomeruli, and renal vesicles were measured (n=6) ; C-D, One male Mongolian gerbil was randomly selected at each week of age, and the area of all renal corpuscles, glomeruli, and renal vesicle of on the largest longitudinal section of the kidneys was measured. 236, 302 and 298 sets of renal corpuscles, glomeruli, and renal vesicles were measured at 12 weeks, 48 weeks, and 72 weeks respectively. The ratio of glomeruli to renal corpusde area was calculated.

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不同周龄段雄性长爪沙鼠肾小体的形态变化

Morphological Changes of Renal Corpuscles in Male Mongolian Gerbils at Different Ages

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