曲古抑菌素A在体细胞核移植中的应用

doi:10.3969/j.issn.1674-5817.2016.02.017

摘要/Abstract

摘要： 体细胞核移植(SCNT)技术已成功获得多种哺乳动物的克隆后代,但一直以来克隆的成功率都很低,并且存活个体大多表型异常。研究表明,重构胚的重编程不完全是导致克隆动物存活率低下和发育异常的主要原因之一。DNA甲基化、组蛋白乙酰化都是影响重编程的重要因素,曲古抑菌素A(TSA)是链霉素的代谢产物,同时也是一种组蛋白去乙酰化酶抑制剂,能降低DNA甲基化水平,促进重编程率,进而提高克隆效率。本文对TSA的生物学功能及其在动物SCNT中的应用进行了探究。

关键词: 曲古抑菌素A(TSA), 体细胞核移植(SCNT), 组蛋白乙酰化

Abstract: Somatic cell nuclear transfer (SCNT) technique has been successfully applied to obtain many mammal clonal offspring. But the success rate was always very low and most surviving individuals showed phenotypic abnormalities. Research shows that the incomplete reprogramming of reconstructed embryo is one of the main reasons that causes the low survival rate and offspring dysplasia. DNA methylation and histone acetylation are both the essential factors that influence the reprogramming. Trichostatin A (TSA ) is a streptomycin metabolite, as well the histone acetylation enzyme inhibitor that reduces the level of DNA methylation and promotes the reprogramming rate to improve the cloning efficiency. The article studies the biological function of TSA and its application in animal SCNT.

Key words: Trichostatin A (TSA ), Somatic cell nuclear transfer (SCNT), Histone acetylation

中图分类号:

Q95-33

施超,陈国强,马育芳. 曲古抑菌素A在体细胞核移植中的应用[J]. 实验动物与比较医学, 2016, 36(2): 157-160. DOI: 10.3969/j.issn.1674-5817.2016.02.017.

SHI Chao,CHEN Guo-qiang,MA Yu-fang. The Application of Trichostatin A for Somatic Cell Nuclear Transfer[J]. Laboratory Animal and Comparative Medicine, 2016, 36(2): 157-160. DOI: 10.3969/j.issn.1674-5817.2016.02.017.

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