家兔宫颈注射荧光纳米颗粒盆腔淋巴结聚集性的实验观察

›› 2010, Vol. 30 ›› Issue (3): 180-182.

家兔宫颈注射荧光纳米颗粒盆腔淋巴结聚集性的实验观察

复旦大学附属妇产科医院，上海 200011

收稿日期:2010-05-12 出版日期:2010-03-31 发布日期:2010-03-31

Observation on Pelvic Lymph Nodes Aggregation by Cervical Injection of Fluorescent Nanoparticles in Rabbits

Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China

Received:2010-05-12 Online:2010-03-31 Published:2010-03-31

摘要/Abstract

摘要： 目的探讨NaYF₄纳米颗粒宫颈注射后在家兔盆腔淋巴结聚集情况，并探讨聚集的最佳直径和最佳时间点.方法 10%NaYF₄纳米颗粒（Ф20、50、100 nm）溶液注射家兔宫颈，注射后（2、10、30、60、120 min）分别取盆腔淋巴结制成切片，观察荧光颗粒的聚集情况，并用浓硝酸消化组织后定量检测NaYF₄纳米颗粒浓度；10%50 nm NaYF₄纳米颗粒溶液注射家兔宫颈10 min后取盆腔淋巴结，肝脏、肾脏和脾脏，并用浓硝酸消化组织后定量检测NaYF₄纳米颗粒浓度.结果 50 nm是NaYF₄纳米颗粒溶液注射家兔宫颈在盆腔淋巴结聚集的最佳直径，10 min是最佳时间点；家兔盆腔淋巴结中NaYF₄纳米颗粒的量大于肝、脾和肾等脏器的量.结论 NaYF₄纳米颗粒溶液经宫颈注射后能够在家兔的盆腔淋巴结中富集.

关键词: NaYF₄, 纳米颗粒, 家兔模型, 生殖器肿瘤, 淋巴结转移, 淋巴富集

Abstract: Objective To investigate whether there is enrichment of NaYF₄ nano-particles in pelvic lymph node by cervical injection in rabbits, and also to decide the peak time and the best diameter of nano-particles. Methods 10% solution with different diameters of nano-particles (20、50、100 nm) is injected through female rabbit's cervix. The pelvic lymph nodes were be taken at different time (2、10、30、60、120 min) after injection and the aggregation peak of nano-particles were measured;inlO min 50 nm group, the liver、spleen and kidneys are taken with pelvic lymph nodes together to detect the aggregation characteristics of nano-particles. Result The 50 nm is a preferable NaYF₄ nano-particles diameter for NaYF₄ solution injection through cervix，and 10 min is the peak time.Compared with the liver，spleen and kidneys，more nano-particles were aggregated in pelvic lymph nodes. Conclusion NaYF₄ nanoparticle solution through cervical injection of the pelvic lymph nodes in rabbits can be enriched.

Key words: NaYF₄ Nano-particles, Rabbit model, Genital cancer, Lymph node metastasis;Lymph concentration

丰华，程明军，徐丛剑. 家兔宫颈注射荧光纳米颗粒盆腔淋巴结聚集性的实验观察[J]. , 2010, 30(3): 180-182.

FENG Hua,CHENG Ming-jun,XU Cong-jian. Observation on Pelvic Lymph Nodes Aggregation by Cervical Injection of Fluorescent Nanoparticles in Rabbits[J]. , 2010, 30(3): 180-182.

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