束缚应激致亚健康状态大鼠氧自由基变化及中药干预作用

›› 2010, Vol. 30 ›› Issue (2): 121-123.

束缚应激致亚健康状态大鼠氧自由基变化及中药干预作用

浙江中医药大学动物实验研究中心/比较医学研究中心，杭州 310053

收稿日期:2009-12-07 出版日期:2010-02-28 发布日期:2010-02-28
基金资助:
1.浙江省中医“治未病”研究专项（2007WB022）；2.“浙江省卫生高层次创新人才培养工程项目”

Oxygen Radical Changes of Sub-healthy Rat Induced by Restraint Stress and Altered by Traditional Chinese Medicine

Zhejiang Chinese Medical University Laboratory Animal Research Center, Hangzhou 310053, China

Received:2009-12-07 Online:2010-02-28 Published:2010-02-28

摘要/Abstract

摘要： 目的限制大鼠活动致束缚应激形成大鼠亚健康状态模型，观察亚健康状态大鼠氧自由基变化及中药干预效果.方法雄性SD大鼠40只，体重320～360 g，随机分成4组，即正常对照组、模型对照组、抗衰老片组和六味地黄丸组，每组10只.除正常组外，其它各组每天限制大鼠活动3 h，形成束缚应激，建立亚健康状态大鼠模型.在造模同时，各给药组灌胃给予相应的药物.试验第14 d和21 d，各组随机取5只大鼠，经腹腔取静脉血，测定SOD、GSH-Px活性和MDA、LD含量.结果模型对照组大鼠血清中SOD、GSH-Px活性明显降低(P＜0.05，P＜0.01)，MDA、LD含量明显升高(P＜0.05);给予抗衰老片后显著提高大鼠血清中SOD、GSH-Px活性和降低血清MDA、LD含量(P＜0.05，P＜0.01).结论连续每日限制活动3 h可使大鼠产生束缚应激，导致抗氧化能力降低，这可能与亚健康状态的形成有关.给予抗衰老片和六味地黄丸可明显提高血清中SOD、GSH-Px活性和降低MDA、LD含量.

关键词: 束缚应激, 亚健康, 抗衰老片, 六味地黄丸, 抗氧化

Abstract: Objective To observe the oxygen free radical change in sub-healthy rat induced by restraint stress and to explore the mechanism of Traditional Chinese Medicine alters the stress.Methods Fourty Male SD rats, weighted 320～360g, were divided randomly into 4 groups, which including normal control group, model control group, anti-aging tablets group and Liuweidihuang pills group, To simulate human physiological stress, the sub-healthy rat model was induced by restraint，meanwhile, the rats in anti-aging tablets group were administrated by anti-aging tablets and Liuweidihuang pills group were treated by Liuweidihuang pills separately. Fourteen and 21 days after experiment，5 rats were selected randomly in each group to collect vein blood in order to test the activities of SOD,GSH-Px and the content MDA、LD. Results The activity of SOD and GSH-Px in blood serum in model rats lowered obviously (P<0.05, P<0.01) while the MDA、LD ascended significantly (P<0.05). The activity of SOD and GSH-Px in serum increased and the content of serum MDA、LD decreased significantly in anti-aging tablets group (P<0.05, P<0.01). Conclusion The restraint stress for 3 hours everyday which could initiate the sub-healthy syndrome, and the antioxidation of model rat was decreased.The treatment of anti-aging tablets and Liuweidihuang pills could improve the sub-healthy state and the mechanism may be related to the effects on antioxidation.

Key words: Restraint stress, Sub-health rats, Anti-aging tablet, Liuweidihuang pills;Free radicals metabolism

谢日青，冷晓霞，陈民利，朱科燕，林琳，陶涛，余佳，潘永明. 束缚应激致亚健康状态大鼠氧自由基变化及中药干预作用[J]. , 2010, 30(2): 121-123.

XIE Ri-qing,LENG Xiao-xia,CHEN Min-li,ZHU Ke-yan,LIN Lin,TAO Tao,YU Jia,PAN Yong-ming. Oxygen Radical Changes of Sub-healthy Rat Induced by Restraint Stress and Altered by Traditional Chinese Medicine[J]. , 2010, 30(2): 121-123.

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