中国灰仓鼠气管和回盲部细菌分离鉴定及药敏试验

›› 2009, Vol. 29 ›› Issue (2): 93-99.

所属专题：实验动物资源开发与利用

中国灰仓鼠气管和回盲部细菌分离鉴定及药敏试验

1.中国药品生物制品检定所，北京，100050；2.首都医科大学附属北京佑安医院，北京，100069

收稿日期:2008-08-20 出版日期:2009-02-28 发布日期:2009-02-28

Identification and Susceptibility Test of Bacteria Isolated from the Trachea and Ileocecal Junction of Grey Hameters from Xinjiang Area of China

1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China;2. Capital University of Medical Science Affiliated Beijing Youan Hospital, Beijing 100069, China)

Received:2008-08-20 Online:2009-02-28 Published:2009-02-28

摘要/Abstract

摘要： 目的对新疆灰仓鼠气管和回盲部携带的细菌种群分布情况及耐药性进行调查分析。方法对新疆灰仓鼠采样进行细菌学分离和生化鉴定，并选择主要细菌进行药敏试验。结果共分离到22株细菌。结果表明，中国灰仓鼠气管和回盲部携带的细菌种群主要有埃希菌属（大肠埃希氏菌）、假单胞菌属（铜绿假单胞菌）、葡萄球菌属（松鼠葡萄球菌）、巴斯德氏菌属（嗜肺巴斯德氏菌、产气巴斯德氏菌、多杀巴斯德氏菌）和芽孢杆菌属（短小芽孢杆菌、蜂房芽孢杆菌、地衣芽孢杆菌、蜡样芽孢杆菌）等，这些菌株对四环素、左氧沙星等药物有很高的敏感性，而对氨苄西林、青霉素G等药物不敏感。结论中国灰仓鼠携带的细菌种群具有多样性，研究结果为中国灰仓鼠的细菌学检测以及质量标准的制定提供了科学依据。

关键词: 中国灰仓鼠, 细菌分离, 鉴定, 药敏试验

Abstract: Objective To investigate the prevalence and the antibiotic resistance of bacteria isolated from the trachea and ileocecal junction of grey hamsters (Cricetulus migratorius) which came from Xinjiang area of China. Methods Twenty two bacterial strains were isolated, which were identified by biochemical assays and were evaluated for resistance to thirty six kinds of antibiotics. Results The identification results showed that the twenty two strains belonged to Escherichia (Escherichia coli), Pseudomonas (Pseudomonas aeruginosa), Staphylococcus (Staphylococcus sciuri), Pasteurella (Pasteurella pneumotropica, Pasteurella aerogenes and Pasteurella multocida) and Bacillus (Bacilluspumilus, Bacillus alvei, Bacillus licheniformis and Bacillus cereus). These bacteria were susceptible to tetracycline and levofloxacin. However, their resistence to ampicillin and penicillin G were found. Conclusion Microbial population carried by grey hamster in China had characters of diversity. Results of this study provided scientifical accordance for the microorganism monitoring of grey hameters in China.

Key words: Cricetulus migratorius., Bacteria Isolation, Identification, Susceptibility Test

高正琴1，贺争鸣1，张强2，岳秉飞1，邢进1，石继春1，冯育芳1，王珊珊1，叶强1. 中国灰仓鼠气管和回盲部细菌分离鉴定及药敏试验[J]. , 2009, 29(2): 93-99.

GAO Zheng-qin1，HE Zheng-ming1，ZHANG Qiang2，YUE Bing-fei1，XING Jin1，SHI Ji-chun1，FENG Yue-fang1，WANG Shan-shan1，YE Qiang1. Identification and Susceptibility Test of Bacteria Isolated from the Trachea and Ileocecal Junction of Grey Hameters from Xinjiang Area of China[J]. , 2009, 29(2): 93-99.

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中国灰仓鼠气管和回盲部细菌分离鉴定及药敏试验

Identification and Susceptibility Test of Bacteria Isolated from the Trachea and Ileocecal Junction of Grey Hameters from Xinjiang Area of China

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