关键词: 辐照剂量率, X射线, 外周血细胞, 免疫器官

Abstract: Objective To explore the effects of different irradiation dose rate of X-ray on mouse peripheral blood cells and immune organ in mouse. Methods Totally 120 mice were randomly divided into 3 groups: the normal control group, low-dose rate irradiated group and high-dose rate irradiated group. The blood samples were taken respectively from mouse of each group at 24 h, 48 h, 96 h, 192 h after irradiation for detecting red blood cells (RBC), white blood cells (WBC), platelets (PLT) and hemoglobins (HGB), and the thymus glands and spleens were taken for calculating organ indexs and determining the content of superoxide dismutase (SOD) and malondialdehyde (MDA). Result The content of RBC, WBC, PLT and HGB in mice were decreased after the different radiation dosages of X-ray, but no significant difference was observed between the high-dose group and the low-dose group. At the same sampling time, a significant difference was observed on the thymus glands index and spleens index of the high-dose and low-dose mice compared with those of the normal control mice. There was a significant difference in the spleens index of the high-dose group and the low-dose group. The thymus glands index had a significant difference between the high-dose group and the low-dose group at the 24 h after irradiation. The contents of MDA were significantly increased, and the contents of SOD were significantly decreased in thymus glands and spleens of the high- and low-dose mice compared with the normal control mice. There was a significant difference in the content of MDA of thymus glands between the high- and low-dose mouse at the sampling time of 48 h and 96 h respectively, while the SOD was observed at the sampling time of 24 h, 48 h and 96 h respectively. There was a significant difference in the content of MDA of spleens between the high- and low-dose mice at sampling time of 24 h and 48 h respectively, while the SOD was observed at the sampling time of 24 h, 48 h and 192 h respectively. Conclusion No significant difference was observed on the effect of high- and low-dose irradiation on the peripheral blood cell in mice. The high-dose irradiation had more influence than low-dose irradiation on the spleen index, while the thymus glands index was not affected by irradiation. The high-dose irradiation had more influence than the low-dose irradiation on the SOD and MDA of the thymus glands and spleens in mice.

Key words: Radiation dose rate, X-ray, Peripheral blood cell, Immune organs