紫杉醇缓释微球对卵巢癌裸小鼠原位移植瘤的抑瘤效果

doi:10.3969/j.issn.1674-5817.2017.02.010

实验动物与比较医学 ›› 2017, Vol. 37 ›› Issue (2): 136-139.DOI: 10.3969/j.issn.1674-5817.2017.02.010

紫杉醇缓释微球对卵巢癌裸小鼠原位移植瘤的抑瘤效果

饶子亮¹, 王弋¹, 王诺¹, 熊仁清², 张明浩², 邝少松¹, 唐小江¹

1.广东省医学实验动物中心, 佛山528248;
2.天大药业(珠海)有限公司, 珠海519070

收稿日期:2016-11-14 出版日期:2017-04-25 发布日期:2017-04-25
作者简介:饶子亮(1983-),男,副主任药师,从事抗肿瘤药物筛选研究。E-mail:343806059@qq.com
基金资助:
广东省科技计划项目(2016A030303027、2013B060300033、2016A030303026)

Inhibition of Paclitaxel-Poly Lactic Acid Phosphate(PLCE) Microspheres on Growth of Ovarian Cancer Transplanted in Nude Mice

RAO Zi-liang¹, WANG Yi¹, WANG Nuo¹, XIONG Ren-qing², ZHANG MING-hao², LOU Cai-xia¹, KUANG Shao-song¹, TANG Xiao-jiang¹

1. Guang Dong Medical Laboratory Animal Center, Foshan 528248, China;
2. Tianda Pharmaceuticals Limited, Zhuhai 519070, China

Received:2016-11-14 Online:2017-04-25 Published:2017-04-25

摘要/Abstract

摘要： 目的研究以新型聚乳酸磷酸酯聚合物(PLCE)为载体的紫杉醇缓释微球体内抑瘤效果。方法采用HO8910人卵巢癌细胞株,手术接种于68只BALB/c裸小鼠卵巢包膜下。3周后,取肿块大小相对均匀的42只裸小鼠随机分成7组: 空白对照组、聚合物空白微球对照组、紫杉醇注射液组,紫杉醇缓释微球1中、低剂量组,紫杉醇缓释微球2中、低剂量组,每组6只。分组当日给药1次,连续观察一个月。结果紫杉醇注射液抑瘤率57.0%; 紫杉醇缓释微球2中剂量抑瘤率77.1%,低剂量抑瘤率58.7%。结论以新型PLCE为载体的紫杉醇缓释微球2(60 mg/kg)对卵巢癌裸小鼠原位移植瘤有良好的抑制效果,但是毒性仍然较大,需要进一步改进。

关键词: 紫杉醇, 微球, 卵巢癌, 聚乳酸磷酸酯共聚物(PLCE)

Abstract: Objective To observe the antitumor effect of paclitaxel- novel copolymer of poly lactic acid phosphate (PLCE) microspheres on proliferation of ovarian cancer. Methods The human overiam cancer (HO8910) were implanted into the ovarian capsule of 68 nude mice. Three weeks later, 42 tumor bearing nude mice were randomly divided into 7 groups as follow, blank control group, negative control group, paclitarel injection group, paclitaxel-PLCE microspheres 1 high and low dose group, and paclitaxel-PLCE microspheres 2 high and low dose group, with 6 nude mice in each group. The drug was administered 1 time a day, and the growth of the tumor were continuously observed for one month. Results The tumor inhibition rate of paclitarel injection was 57.0%, and tumor inhibition rate of paclitaxel-PLCE microspheres 2 high and low dose group were 77.1% and 58.7% respectively. Conclusion Paclitaxel-PLCE microspheres have a strong inhibitory effect on HO8910 ovarian cancer transplanted in nude mice.

Key words: Paclitaxel, Microspheres, Ovarian cancer, Poly lactic acid phosphate (PLCE)

中图分类号:

R965.1Q95-33

饶子亮, 王弋, 王诺, 熊仁清, 张明浩, 邝少松, 唐小江. 紫杉醇缓释微球对卵巢癌裸小鼠原位移植瘤的抑瘤效果[J]. 实验动物与比较医学, 2017, 37(2): 136-139.

RAO Zi-liang, WANG Yi, WANG Nuo, XIONG Ren-qing, ZHANG MING-hao, LOU Cai-xia, KUANG Shao-song, TANG Xiao-jiang. Inhibition of Paclitaxel-Poly Lactic Acid Phosphate(PLCE) Microspheres on Growth of Ovarian Cancer Transplanted in Nude Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(2): 136-139.

参考文献

[1] Vaughn DJ, Brown AW Jr, Harker WC, et al.Multicenter phase II study of estramustine phosphate plus weekly paclitaxel in patientes with androgen-independent prostate carcinoma[J]. Cancer, 2004, 100(4):746-750.
[2] 张烁, 郭文彬, 朱梅. 载紫杉醇缓释微球的制备及其体外抑瘤效果的观察[J]. 山东大学学报: 医学版, 2011, 49(12):39-47.
[3] 王美清. 紫杉醇的临床毒副作用分析及对策[J]. 中国热带医学, 2007, 7(2):237-239.
[4] Ruan G, Feng SS.Preparation and characterization of poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PLA-PEC-PLA) micro-spheres for controlled release of paclitaxel[J]. Biomaterials, 2003, 24(27):5037-5044.
[5] Liu Z, Zhu YY, Li ZY, et al.Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells[J]. Oncol Lett, 2016, 12(5):3944-3948.
[6] Tyulyandina A, Harrison D, Yin W, et al. Alofanib, an allosteric FGFR2 inhibitor, has potent effects on ovarian cancer growth in preclinical studies[J]. Invest New Drugs, 2016(3). [Epub ahead of print]
[7] Ai B, Bie Z, Zhang S, et al.Paclitaxel targets VEGF-mediated angiogenesis in ovarian cancer treatment[J]. Am J Cancer Res, 2016, 6(8):1624-1635.
[8] Osman MA, Elkady MS, Nasr KE.Weekly Paclitaxel versus three-weekly Paclitaxel in recurrent platinum-resistant epithelialovarian and peritoneal cancers: A phase iii study[J]. Clin Med Insights Oncol, 2016, 10:35-41.
[9] Ishiguro K, Zhu YL, Lin ZP, et al.Cataloging antineoplastic agents according to their effectiveness against platinum-resistant and platinum-sensitive ovarian carcinoma cell lines[J]. J Transl Sci, 2016, 2(2):117-124.
[10] 余志英, 李丽文, 杜静, 等. 紫杉醇诱导卵巢癌细胞凋亡及细胞周期改变的研究[J]. 中国医师杂志, 2006, 18(3):322-324.

紫杉醇缓释微球对卵巢癌裸小鼠原位移植瘤的抑瘤效果

Inhibition of Paclitaxel-Poly Lactic Acid Phosphate(PLCE) Microspheres on Growth of Ovarian Cancer Transplanted in Nude Mice

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