Preliminary Study on Raising and Maintaining Clean Mice with Micro-barrier Cages in Conventional Environmental Facilities

doi:10.12300/j.issn.1674-5817.2022.129

Abstract

Abstract:

Objective To explore the feasibility of breeding cleaning degree mice with micro-barrier cages in conventional environmental facilities. Methods Clean-grade mice were housed and maintained in positive-pressure microbarrier cages in a general environment, during which third-party sampling of the microbial status of the animals in the microbarrier was performed on a quarterly frequency. Results From June 2020 to July 2022, no microorganisms that must be excluded by the national standard for cleaning degree mice have been detected in two consecutive years. Conclusion Micro-barrier cages can be used to raise and maintain cleaning grade mice in conventional environmental facilities.

Key words: Micro-barrier cage, Cleaning degree mice, Breeding management

CLC Number:

Q95-33

Qi REN,Yandong LI,Qiang SUN. Preliminary Study on Raising and Maintaining Clean Mice with Micro-barrier Cages in Conventional Environmental Facilities[J]. Laboratory Animal and Comparative Medicine, 2022, 42(5): 440-447. DOI: 10.12300/j.issn.1674-5817.2022.129.

Figures/Tables 6

References 7

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项目 Item	参数 Parameter	参考值（国家标准屏障环境） Reference（GB barrier environment）
换气次数/(次·h^-1) Number of air exchanges/(time·h^-1)	15～100（可调）	≥15
气流速度/(m·s^-1) Air velocity/(m·s^-1)	≤0.1	≤0.2
饲养仓静压差/Pa Static pressure difference/Pa	≥15	≥15
洁净度/级 Cleanliness/grade	小于7级	7级
菌下落数/CFU Number of bacterial colonies/CFU	≤3	≤3
噪声/db Noise/db	≤60	≤60
动物照度/lx Animal illumination/lx	15～20（可调）	15～20

项目 Item	参数 Parameter	参考值（国家标准屏障环境） Reference（GB barrier environment）
换气次数/(次·h^-1) Number of air exchanges/(time·h^-1)	15～100（可调）	≥15
气流速度/(m·s^-1) Air velocity/(m·s^-1)	≤0.1	≤0.2
饲养仓静压差/Pa Static pressure difference/Pa	≥15	≥15
洁净度/级 Cleanliness/grade	小于7级	7级
菌下落数/CFU Number of bacterial colonies/CFU	≤3	≤3
噪声/db Noise/db	≤60	≤60
动物照度/lx Animal illumination/lx	15～20（可调）	15～20

检查项目Item	2020年		2021年				2022年
检查项目Item	Q3	Q4	Q1	Q2	Q3	Q4	Q1	Q2
仙台病毒（SV）
鼠痘病毒 (ECTV)
小鼠肝炎病毒 (MHV)
小鼠肺炎病毒 (PVM)
呼肠孤病毒 (Reo-3)
小鼠细小病毒 (MVM)
沙门氏菌 (SALM)
泰泽病原体 (CPIL)
支原体 (MYCO)
鼠棒状杆菌 (CKUT)
嗜肺巴斯德杆菌 (PPNE)					●	▲●	▲●	●
肺炎克雷伯杆菌 (KPNE)
金黄色葡萄球菌 (SAUR)
绿脓杆菌 (PAER)						▲	▲	●
体外寄生虫 (ECTO)
肠道蠕虫 (HELM)
弓形虫 (TOXO)
鞭毛虫 (FLAG)
纤毛虫 (CILI)

检查项目Item	2020年		2021年				2022年
检查项目Item	Q3	Q4	Q1	Q2	Q3	Q4	Q1	Q2
仙台病毒（SV）
鼠痘病毒 (ECTV)
小鼠肝炎病毒 (MHV)
小鼠肺炎病毒 (PVM)
呼肠孤病毒 (Reo-3)
小鼠细小病毒 (MVM)
沙门氏菌 (SALM)
泰泽病原体 (CPIL)
支原体 (MYCO)
鼠棒状杆菌 (CKUT)
嗜肺巴斯德杆菌 (PPNE)					●	▲●	▲●	●
肺炎克雷伯杆菌 (KPNE)
金黄色葡萄球菌 (SAUR)
绿脓杆菌 (PAER)						▲	▲	●
体外寄生虫 (ECTO)
肠道蠕虫 (HELM)
弓形虫 (TOXO)
鞭毛虫 (FLAG)
纤毛虫 (CILI)

时间段 Time period		屏障饲养区 Barrier breeding area		微屏障饲养区 Micro barrier breeding area
时间段 Time period		实验人员总数 Total number of laboratory personnel	人员进出次数 Number of personnel in and out	实验人员总数 Total number of laboratory personnel	人员进出次数 Number of personnel in and out
2020年	Q3	12	93	30	786
2020年	Q4	14	94	46	927
2021年	Q1	9	102	29	690
	Q2	7	59	34	726
	Q3	18	151	37	687
	Q4	13	103	37	760
2022年	Q1	13	105	39	764
2022年	Q2	4	30	42	801