Improvement of Detection Method of Pasteurella pneumotropica and Its Medical Elimination in Mice

doi:10.12300/j.issn.1674-5817.2020.197

Abstract

Abstract: Objective To modify the method for sensitive detection of Pasteurella pneumotropica in mice in routine monitoring, and to explore the feasibility of medical elimination in Pasteurella pneumotropica infected mice.Methods The Pasteurella pneumotropica were detected by routine procedure and modified method that using fluid medium before inoculation. The detection rates of the two methods were compared in statistics. The sensitivity of Pasteurella pneumotropica isolated from local facility to 12 antibiotics was studied by antimicrobial disk sensitivity test. Forty-eight female C57BL/6 mice were randomly divided into 6 groups, with 8 in each group. All the groups were set to different concentration of enrofloxacin continuously for 6 weeks to find out the safe dosage. Mice were infected with local Pasteurella pneumotropica strain, the Pasteurella pneumotropica positive mice were administered enrofloxacin in drinking water at different doses to evaluate the effect of Pasteurella pneumotropica clearance results.Results The detection rates of Pasteurella pneumotropica by routine procedure and improved method were 1.3% and 15.1%, respectively. Pasteurella pneumotropica was sensitive to enrofloxacin, cefoxitin and cefadrazole etc. The mice were safe to enrofloxacin within a daily dosage of 300 mg/kg. The Pasteurella pneumotropica was not detected from positive mice treated with 85 mg/kg enrofloxacin after 2 weeks, and kept negative after withdrawl for 6 weeks.Conclusion Using fluid medium before inoculation can improve the positive detection rate of Pasteurella pneumotropica, which is simple, convenient, and suitable for daily health monitoring of animal facility. Oral administration of enrofloxacin more than a dosage of 85 mg·kg^–1·d^–1 can elimilate Pasteurella pneumotropica from the positive mice safely and effectively, and be an alternative contingency plan for the elimination of Pasteurella pneumotropica in mice.

Key words: Pasteurella pneumotropica, Detection method improvement, Enrofloxacin, Antimicrobial susceptibility test, Medical elimination

CLC Number:

Q95-33
R-332

ZHANG Tao, CUI Can, MA Guanzhong, ZHANG Aihua. Improvement of Detection Method of Pasteurella pneumotropica and Its Medical Elimination in Mice[J]. Laboratory Animal and Comparative Medicine, 2021, 41(4): 351-357.

Figures/Tables 5

References

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