Improvement of Detection Method of Pasteurella pneumotropica and Its Medical Elimination in Mice

doi:10.12300/j.issn.1674-5817.2020.197

Abstract

Abstract: Objective To modify the method for sensitive detection of Pasteurella pneumotropica in mice in routine monitoring, and to explore the feasibility of medical elimination in Pasteurella pneumotropica infected mice.Methods The Pasteurella pneumotropica were detected by routine procedure and modified method that using fluid medium before inoculation. The detection rates of the two methods were compared in statistics. The sensitivity of Pasteurella pneumotropica isolated from local facility to 12 antibiotics was studied by antimicrobial disk sensitivity test. Forty-eight female C57BL/6 mice were randomly divided into 6 groups, with 8 in each group. All the groups were set to different concentration of enrofloxacin continuously for 6 weeks to find out the safe dosage. Mice were infected with local Pasteurella pneumotropica strain, the Pasteurella pneumotropica positive mice were administered enrofloxacin in drinking water at different doses to evaluate the effect of Pasteurella pneumotropica clearance results.Results The detection rates of Pasteurella pneumotropica by routine procedure and improved method were 1.3% and 15.1%, respectively. Pasteurella pneumotropica was sensitive to enrofloxacin, cefoxitin and cefadrazole etc. The mice were safe to enrofloxacin within a daily dosage of 300 mg/kg. The Pasteurella pneumotropica was not detected from positive mice treated with 85 mg/kg enrofloxacin after 2 weeks, and kept negative after withdrawl for 6 weeks.Conclusion Using fluid medium before inoculation can improve the positive detection rate of Pasteurella pneumotropica, which is simple, convenient, and suitable for daily health monitoring of animal facility. Oral administration of enrofloxacin more than a dosage of 85 mg·kg^–1·d^–1 can elimilate Pasteurella pneumotropica from the positive mice safely and effectively, and be an alternative contingency plan for the elimination of Pasteurella pneumotropica in mice.

Key words: Pasteurella pneumotropica, Detection method improvement, Enrofloxacin, Antimicrobial susceptibility test, Medical elimination

CLC Number:

Q95-33
R-332

ZHANG Tao,CUI Can,MA Guanzhong,et al. Improvement of Detection Method of Pasteurella pneumotropica and Its Medical Elimination in Mice[J]. Laboratory Animal and Comparative Medicine, 2021, 41(4): 351-357. DOI: 10.12300/j.issn.1674-5817.2020.197.

Figures/Tables 5

References

[1] 邢进, 冯育芳, 岳秉飞, 等. 嗜肺巴斯德杆菌研究进展[J]. 中国实验动物学报, 2014, 22(2):90-94. DOI:10.3969/j.issn.1005-4847.2014.02.0020.
[2] 田克恭, 贺争鸣, 刘群. 实验动物疫病学[M]. 北京: 中国农业出版社, 2015.
[3] UENO Y, SHIMIZU R, NOZU R, et al.Elimination of Pasteurella pneumotropica from a contaminated mouse colony by oral administration of Enrofloxacin[J]. Exp Anim, 2002, 51(4):401-405. DOI:10.1538/expanim.51.401.
[4] 中华人民共和国国家质量监督检验检疫总局. 实验动物嗜肺巴斯德杆菌检测方法: GB/T 14926.12—2001[S]. 北京: 中国标准出版社, 2002.
[5] Duellet M, Cowan M, Laporte A,et al.Implementation of a PCR assay of Pasteurella pneumotropica to accurately screen for contaminated laboratory mice[J]. Lab Anim, 2011, 40(10):305-312. DOI:10.1038/laban 1011-305.
[6] 赵莹莹, 邢卉春. 基于不同引物的16S rRNA序列分析法对SBP腹水细菌的鉴定及抗感染疗效评价的意义[J]. 世界华人消化杂志, 2015, 23(29):4713-4719. DOI:10.11569/wcjd.v23.i29.4713.
[7] 王冬平, 李善如, 张敏, 等. 三种小鼠血液生理生化正常值的测定[J]. 实验动物科学与管理, 2000, 17(2):24-28. DOI:CNKI:SUN:SYDG.0.2000-02-005.
[8] FOX J G, ANDERSON L C, OTTO G M, et al.Laboratory animal medicine[M]. 3rd Ed. Amsterdam: Elsevier Inc, 2015:57-58.
[9] GOELZ M F, THIGPEN J E, MAHLER J, et al.Efficacy of various therapeutic regimens in eliminating Pasteurella pneumotropica from the mouse[J]. Lab Anim Sci, 1996, 46(3):280-285.
[10] MATSUMIYA L C, LAVOIE C.An outbreak of Pasteurella pneumotropica in genetically modified mice: treatment and elimination[J]. Contemp Top Lab Anim Sci, 2003, 42(2):26-28.
[11] 李红, 刘星, 张丽芳, 等. 嗜肺巴斯德杆菌选择性培养基研制及应用[J]. 中国实验动物学报, 2001, 9(1):20-25. DOI:10.3969/j.issn.1005-4847.2001.01.004.
[12] 高正琴, 邢进, 王春玲, 等. 小鼠嗜肺巴氏杆菌感染的监测与分析[J]. 实验动物与比较医学, 2007, 27(3):183-185. DOI:10.3969/j.issn.1674-5817.2007.03.009.
[13] 赵晶, 康世良. 兽用喹诺酮类抗菌剂恩诺沙星的药物代谢动力学研究进展[J]. 黑龙江畜牧兽医, 1999(4):40-41. DOI:CNKI:SUN:HLJX.0.1999-04-038.
[14] 董漓波, 叶启薇, 孙永学, 等. 恩诺沙星对SD系大白鼠的急性及亚急性毒性研究[J]. 华南农业大学学报, 1995, 16(4):1-4. DOI:CNKI:SUN:HNNB.0.1995-04-000.

[1]	JIAO Qingzhen, WU Guihua, TANG Wen, FAN Fan, FENG Kai, YANG Chunxiang, QIAO Jian, DENG Sufang. Dynamic Monitoring and Analysis of Ammonia Concentration in Laboratory Animal Facilities Under Suspension of Heating Ventilation and Air Conditioning System [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 490-495.
[2]	LIU Wentao, LUO Yanhong, LONG Yongxia, LUO Qihui, CHEN Zhengli, LIU Lida. Common Environmental Problems and Testing Experiences in Laboratory Animal Facilities in Sichuan Province [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 483-489.
[3]	ZHAO Xin, WANG Chenxi, SHI Wenqing, LOU Yuefen. Advances in the Application of Zebrafish in the Research of Inflammatory Bowel Disease Mechanisms and Drug Development [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 422-431.
[4]	GONG Leilei, WANG Xiaoxia, FENG Xuewei, LI Xinlei, ZHAO Han, ZHANG Xueyan, FENG Xin. A Mouse Model and Mechanism Study of Premature Ovarian Insufficiency Induced by Different Concentrations of Cyclophosphamide [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 403-410.
[5]	LIN Zhenhua, CHU Xiangyu, WEI Zhenxi, DONG Chuanjun, ZHAO Zenglin, SUN Xiaoxia, LI Qingyu, ZHANG Qi. Evaluation of the Safety and Efficacy of Bone Cement in Experimental Pigs Using Vertebroplasty [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 466-472.
[6]	JIANG Juan, SONG Ning, LIAN Wenbo, SHAO Congcong, GU Wenwen, SHI Yan. Comparison of Histopathological and Molecular Pathological Phenotypes in Mouse Models of Intrauterine Adhesions Induced by Two Concentrations of Ethanol Perfusion [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 393-402.
[7]	LIU Yueqin, XUE Weiguo, WANG Shuyou, SHEN Yaohua, JIA Shuyong, WANG Guangjun, SONG Xiaojing. Observation of Digestive Tract Tissue Morphology in Mice Using Probe-Based Confocal Laser Endomicroscopy [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 457-465.
[8]	ZHENG Qingyong, YANG Donghua, MA Zhichao, ZHOU Ziyu, LU Yang, WANG Jingyu, XING Lina, KANG Yingying, DU Li, ZHAO Chunxiang, DI Baoshan, TIAN Jinhui. Recommendations for Standardized Reporting of Systematic Reviews and Meta-Analysis of Animal Experiments [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 496-507.
[9]	WANG Tingjun, LUO Hao, CHEN Qi. Discussion on AI-Based Digital Upgrade and Application Practice of Laboratory Animal Centers [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 473-482.
[10]	WANG Jiaoxiang, ZHANG Lu, CHEN Shuhan, JIAO Deling, ZHAO Heng, WEI Taiyun, GUO Jianxiong, XU Kaixiang, WEI Hongjiang. Construction and Functional Validation of GTKO/hCD55 Gene-Edited Xenotransplant Donor Pigs [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 379-392.
[11]	QIN Chao, LI Shuangxing, ZHAO Tingting, JIANG Chenchen, ZHAO Jing, YANG Yanwei, LIN Zhi, WANG Sanlong, WEN Hairuo. Study on the 90-day Feeding Experimental Background Data of SD Rats for Drug Safety Evaluation [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 439-448.
[12]	LIU Kun, LAN Qing, YI Bing, XIE Xiaojie. Key Challenges and Mitigation Strategies for Animal Pregnancy in Non-clinical Reproductive Toxicity Testing of Drugs [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 449-456.
[13]	. [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 508-514.
[14]	CHEN Ziyi, SUN Hongyan, KANG Pinfang, WU Wenjuan. Research Advances in Animal Experimental Models of Pulmonary Hypertension [J]. Laboratory Animal and Comparative Medicine, 2025, (): 1-12.
[15]	XU Yingtao, WANG Mengmeng, LIN Ping, CHI Haitao, WANG Yi, BAI Ying. Exosomes Improve Ischemic Stroke by Regulation of Ferroptosis Through the NRF2/SLC7A11/GPX4 Pathway in Mice [J]. Laboratory Animal and Comparative Medicine, 2025, (): 1-11.